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A Pathway of Chitosan Formation in Mucor rouxii: Enzymatic Deacetylation of Chitin
Abstract
An enzyme that catalyzes hydrolysis of acetamido groups of chitin derivatives was found in the supernatant fraction of Mucor rouxii. Partially O‐hydroxyethylated chitin (glycol chitin) was used as a substrate in the purification and characterization of this enzyme. A 140‐fold purification was obtained by means of ammonium sulfate fractionation followed by chromatography on carboxymethylcellulose and DEAE‐cellulose. The enzyme releases about 30% of the acetyl groups of glycol chitin, giving a product with a decreased sensitivity to lysozyme. The enzyme also deacetylates chitin and N‐acetylchitooligoses, whereas it is inactive toward bacterial cell wall peptidoglycan, N‐acetylated heparin, a polymer of N‐acetylgalactosamine, di‐N‐acetylchitobiose and monomeric N‐acetylglucosamine derivatives. This enzyme shows a pH optimum of 5.5. The Km value for glycol chitin is 0.87 g/l or 2.6 mM with respect to monosaccharide residues. The occurrence of this enzyme accounts for the formation of chitosan in fungi.
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Cited by
Muzzarelli, R. A. A., Boudrant, J., Meyer, D., Manno, N., DeMarchis, M., & Paoletti, M. G. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.09.063 · Cited by 694
DEVELOPMENTAL REGULATION OF FUNGAL CELL WALL FORMATION
Wessels, J. G. H. (1994) · Annual Review of Phytopathology · doi:10.1146/annurev.py.32.090194.002213 · Cited by 419
Chitin deacetylases: new, versatile tools in biotechnology
Tsigos, I., Martinou, A., Kafetzopoulos, D., & Bouriotis, V. (2000) · Trends in Biotechnology · doi:10.1016/s0167-7799(00)01462-1 · Cited by 381
Vander, P., Vårum, K. M., Domard, A., Eddine El Gueddari, N., & Moerschbacher, B. M. (1998) · Plant Physiology · doi:10.1104/pp.118.4.1353 · Cited by 339
El Ghaouth, A., Arul, J., Asselin, A., & Benhamou, N. (1992) · Mycological Research · doi:10.1016/s0953-7562(09)80447-4 · Cited by 289
Metadata
- Authors
- ARAKI, Y., & ITO, E.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1975-06
- Volume
- 55
- Issue
- 1
- Pages
- 71-78
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2137596882
- Cited by
- 233
- References
- 0
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- closed
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- ARAKI, Y., & ITO, E. (1975). A Pathway of Chitosan Formation in Mucor rouxii: Enzymatic Deacetylation of Chitin. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1975.tb02139.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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