The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography
Abstract
A method is described for the rapid preparation of plasmid DNAs of molecular weight up to 14 X 10(6). This method involves the chromatography, at room temperature, of bacterial cleared lysates on hydroxyapatite in the presence of high concentrations of phosphate and urea. All detectable protein and RNA contamination of plasmid DNA is removed by this procedure and the conformation of the plasmid DNA is unaffected. Less than 0.5% chromosomal DNA is present in the purified preparation and even this can be removed if necessary by a simple extention of the procedure to include a heat-denaturation step. The method is extremely rapid and amenable to large-scale plasmid preparation; 5 mg ColE1 DNA have been purified within 40 min. The yield of plasmid DNA is similar to that obtained with the conventional dye-centrifugation technique, however the purity is greater.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Burton, K. (1956) · Biochemical Journal · doi:10.1042/bj0620315 · Cited by 14,090
Cited by
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,887
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
A rapid boiling method for the preparation of bacterial plasmids
Holmes, D. S., & Quigley, M. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90473-5 · Cited by 3,265
Expression of bacterial genes in plant cells.
Fraley, R. T., Rogers, S. G., Horsch, R. B., Sanders, P. R., Flick, J. S., Adams, S. P., Bittner, M. L., Brand, L. A., Fink, C. L., Fry, J. S., et al. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.15.4803 · Cited by 849
Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli
Kieser, T. (1984) · Plasmid · doi:10.1016/0147-619x(84)90063-5 · Cited by 745
Metadata
- Authors
- COLMAN, A., BYERS, M. J., PRIMROSE, S. B., & LYONS, A.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1978-11
- Volume
- 91
- Issue
- 1
- Pages
- 303-310
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2111729024
- Cited by
- 223
- References
- 1
- FWCI
- 11.44
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography. https://app.cochat.ai/papers/10.1111/j.1432-1033.1978.tb20966.x
- The paper
- COLMAN, A., BYERS, M. J., PRIMROSE, S. B., & LYONS, A. (1978). Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1978.tb20966.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
