The DOI matches its Crossref record.
Comparative Analysis of the Effect of Aminoglycosides on Bacterial Protein Synthesis in vitro
Open accessAbstract
The effect of aminoglycoside antibiotics on bacterial protein synthesis in an initiating system was investigated. Three classes of compounds can be differentiated as to their response. (1) Antibiotics (streptomycin, bluensomycin and hygromycin B) which inhibit polypeptide synthesis in a monophasic way indicating a single major inhibitory site. Polypeptides made under partially inhibitory conditions possess the same size as those produced without the drug. (2) Compounds containing 2‐deoxystreptamine in a diglycosylated form (gentamicins B, C 1, C 1a and C 2; sisomicin; kanamycin; neomycin; tobramycin; lividomycin A and paromomycin) inhibit protein synthesis in the triphasic way recently reported for gentamicin C complex which indicates multiple interactions of these antibiotics with the ribosome. These compounds also share the property of promoting the synthesis of longer than normal peptides due to termination errors. (3) The disaccharidic sisomicin and lividomycin cleavage products, garamine and lividamine respectively, give a biphasic response, namely stimulation of the rate of polypeptide synthesis at low concentration and inhibition at increasing concentrations. Aminoglycosides of class 2 (when present at second‐phase concentration) can revert the inhibition of protein synthesis caused by each of the class 1 compounds. Concomitantly, ‘read‐through’ products are accumulated.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“HygB has a single binding site within the 30S (Figure 1) consistent with the finding that it has a monophasic effect on translation (Zierhut et al. 1979”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Zierhut et al. 1979
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“HygB has a monophasic effect on translation that is indicative of a single binding site on the ribosome (Zierhut et al. 1979”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Zierhut et al. 1979
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
No references could be resolved yet.
Research works printed but not linked: 24
- 10.1016/S0021-9258(18)93308-9 · DOI
- 10.1111/j.1432-1033.1978.tb12347.x · DOI
- 10.1016/0006-291X(78)91493-6 · DOI
- 10.1021/bi00568a029 · DOI
- 10.1021/bi00568a028 · DOI
- 10.1016/0006-291X(78)90178-X · DOI
- 10.1007/BF00455118 · DOI
- 10.1016/0022-2836(72)90108-8 · DOI
- 10.1016/S0022-2836(66)80263-2 · DOI
- 10.1016/0022-2836(72)90329-4 · DOI
- 10.1007/BF00274011 · DOI
- 10.1073/pnas.61.4.1279 · DOI
- 10.1128/AAC.2.4.294 · DOI
- 10.1016/0022-2836(73)90248-9 · DOI
- 10.1111/j.1432-1033.1976.tb10587.x · DOI
- 10.1073/pnas.75.11.5534 · DOI
- Weber K., 1975, RNA Phages, 51
- 10.1073/pnas.51.5.883 · DOI
- Gorini L., 1974, Ribosomes, 791
- 10.1073/pnas.61.2.768 · DOI
- 10.1073/pnas.67.3.1148 · DOI
- 10.7164/antibiotics.25.212 · DOI
- Davies J., 1965, Mol. Pharmacol., 1, 93
- Gale E. F., 1972, The Molecular Basis of Antibiotic Action, 278
Cited by
Sorted by citations
Brodersen, D. E., Clemons, W. M., Carter, A. P., Morgan-Warren, R. J., Wimberly, B. T., & Ramakrishnan, V. (2000) · Cell · doi:10.1016/s0092-8674(00)00216-6 · Cited by 950
“…to render it inactive, or mimic the structure and function of the elongation factors and are thus able to a single binding site on the ribosome (Zierhut et al., 1979) and has been shown to bind close to the A site release the bound antibiotic from the ribosome (Mana-vathu et al., 1990;…”
Background
Metadata
- Authors
- ZIERHUT, G., PIEPERSBERG, W., & BÖCK, A.
- Affiliations
- University of Regensburg (DE)
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1979-08
- Volume
- 98
- Issue
- 2
- Pages
- 577-583
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2074829556
- Cited by
- 35
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 1 · 2019: 1
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
- Keywords
- aminoglycosides, bacterial protein synthesis, ribosome, streptomycin, hygromycin B, gentamicin, sisomicin, kanamycin, neomycin, tobramycin, paromomycin, 2-deoxystreptamine, readthrough, antibiotic binding sites
- MeSH terms
- Aminoglycosides, Bacterial Proteins, Drug Resistance, Microbial, Escherichia coli, Gentamicins, Kinetics, Ribosomes, Streptomycin, Structure-Activity Relationship, Protein Biosynthesis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Comparative Analysis of the Effect of Aminoglycosides on Bacterial Protein Synthesis in vitro. https://app.cochat.ai/papers/10.1111/j.1432-1033.1979.tb13219.x
- The paper
- ZIERHUT, G., PIEPERSBERG, W., & BÖCK, A. (1979). Comparative Analysis of the Effect of Aminoglycosides on Bacterial Protein Synthesis in vitro. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1979.tb13219.x
