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Catalytic activity and denaturation of enzymes in water/organic cosolvent mixtures: α‐Chymotrypsin and laccase in mixed water/alcohol, water/glycol and water/formamide solvents
Open accessAbstract
The dependence of the catalytic activities of α‐chymotrypsin and laccase on the concentration of organic cosolvents (alcohols, glycols and formamides) in mixed aqueous media has a pronounced threshold character: it does not change up to a critical concentration of the non‐aqueous cosolvents added, yet further increase of the latter (by only a small percentage, by vol.) leads to an abrupt decrease in enzyme activity. Fluorescence studies indicate that the inactivation results from reversible conformational changes (denaturation) of the enzymes. There is a linear correlation between the critical concentration of residual water (at which the enzyme inactivation occurs in a threshold manner) and the hydrophobicity of the organic cosolvents added. A quantitative criterion is suggested for the selection of organic cosolvents to be used for enzymatic reactions in homogeneous water/organic solvent media.
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Cited by
Strategies for Stabilization of Enzymes in Organic Solvents
Stepankova, V., Bidmanova, S., Koudelakova, T., Prokop, Z., Chaloupkova, R., & Damborsky, J. (2013) · ACS Catalysis · doi:10.1021/cs400684x · Cited by 659
Zhuo, R., Zhang, J., Yu, H., Ma, F., & Zhang, X. (2019) · Chemosphere · doi:10.1016/j.chemosphere.2019.06.113 · Cited by 75
Metadata
- Authors
- MOZHAEV, V. V., KHMELNITSKY, Y. L., SERGEEVA, M. V., BELOVA, A. B., KLYACHKO, N. L., LEVASHOV, A. V., & MARTINEK, K.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1989-10
- Volume
- 184
- Issue
- 3
- Pages
- 597-602
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1565644117
- Cited by
- 217
- References
- 0
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Catalytic activity and denaturation of enzymes in water/organic cosolvent mixtures: α‐Chymotrypsin and laccase in mixed water/alcohol, water/glycol and water/formamide solvents. https://app.cochat.ai/papers/10.1111/j.1432-1033.1989.tb15055.x
- The paper
- MOZHAEV, V. V., KHMELNITSKY, Y. L., SERGEEVA, M. V., BELOVA, A. B., KLYACHKO, N. L., LEVASHOV, A. V., & MARTINEK, K. (1989). Catalytic activity and denaturation of enzymes in water/organic cosolvent mixtures: α‐Chymotrypsin and laccase in mixed water/alcohol, water/glycol and water/formamide solvents. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1989.tb15055.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
