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Dietary protein concentration affects intestinal microbiota of adult cats: a study using DGGE and qPCR to evaluate differences in microbial populations in the feline gastrointestinal tract
Abstract
Summary: The objective of this study was to identify qualitative and quantitative differences in microbial populations of adult cats fed diets containing different protein concentrations. Following a 4 week baseline period, eight healthy adult domestic short‐hair queens (>1‐year‐old) were randomly allotted to a moderate‐protein (MP;n = 4) or high‐protein (HP;n = 4) diet for 8 weeks. Fresh faecal samples were collected after baseline and 8 weeks on treatment and stored at −80 °C. Following DNA extraction, samples were analyzed using denaturing gradient gel electrophoresis to distinguish qualitative changes between diets. Quantitative polymerase chain reaction was used to measure E. coli,Bifidobacterium, Clostridium perfringens,and Lactobacillus populations. Compared to baseline, cats fed MP had a bacterial similarity index of 66.7% as opposed to 40.6% similarity for those fed HP, exhibiting marked changes in intestinal bacteria of cats fed HP.Bifidobacterium populations were greater (p < 0.05) in cats fed MP versus HP (9.44 vs. 5.63 CFU/g).Clostridium perfringens populations were greater (p < 0.05) in cats fed HP than MP (12.39 vs. 10.83 CFU/g). In this experiment, a high‐protein diet resulted in a dramatic shift in microbial populations. Decreased Bifidobacterium population in cats fed HP may justify prebiotic supplementation for such diets.
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“Microbial population shifts in response to dietary protein load have been demonstrated in cats [16] and dogs [17], [20].”
From Using Gross Energy Improves Metabolizable Energy Predictive Equations for Pet Foods Whereas Undigested Protein and Fiber Content Predict Stool Quality (2013) · cites this paper as [16]
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“Previous studies have suggested that an overabundance of protein in the diet may be a negative factor for stool quality, whereas dietary fiber is a positive factor [16]–[19].”
From Using Gross Energy Improves Metabolizable Energy Predictive Equations for Pet Foods Whereas Undigested Protein and Fiber Content Predict Stool Quality (2013) · cites this paper as [16]
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Cited by
Jang, L.-G., Choi, G., Kim, S.-W., Kim, B.-Y., Lee, S., & Park, H. (2019) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-019-0290-y · Cited by 224
Hall, J. A., Melendez, L. D., & Jewell, D. E. (2013) · PLoS ONE · doi:10.1371/journal.pone.0054405 · Cited by 92
Liu, Z.-M., Xu, Z.-Y., Han, M., & Guo, B.-H. (2015) · International Dairy Journal · doi:10.1016/j.idairyj.2014.08.009 · Cited by 29
Evaluation of two raw diets vs a commercial cooked diet on feline growth
Hamper, B. A., Bartges, J. W., & Kirk, C. A. (2017) · Journal of Feline Medicine and Surgery · doi:10.1177/1098612x16634388 · Cited by 17
Metadata
- Authors
- Lubbs, D. C., Vester, B. M., Fastinger, N. D., & Swanson, K. S.
- Venue
- Journal of Animal Physiology and Animal Nutrition
- Publisher
- Wiley
- Published
- 2009-01-07
- Volume
- 93
- Issue
- 1
- Pages
- 113-121
- ISSN
- 0931-2439
- 1439-0396
- Cited by
- 103
- References
- 0
- Language
- en
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- Lubbs, D. C., Vester, B. M., Fastinger, N. D., & Swanson, K. S. (2009). Dietary protein concentration affects intestinal microbiota of adult cats: a study using DGGE and qPCR to evaluate differences in microbial populations in the feline gastrointestinal tract. Journal of Animal Physiology and Animal Nutrition. https://doi.org/10.1111/j.1439-0396.2007.00788.x
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