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Molecular diagnostics of clinical strains of filamentous Basidiomycetes: Molekulare Diagnostik klinischer Stämme filamentöser Basidiomyzeten
Abstract
Summary. Eleven clinical and veterinary strains of filamentous Basidiomycetes were compared with 15 reference strains representing the orders Aphyllophorales and Agaricales. The methods used were restriction analysis of small subunit (18S) (SSU) rDNA and internal transcribed spacers (ITS) 1 and 2 and variable domain 9 (V9)‐ITS1 sequencing. Six strains were found to belong to the teleomorph genera Schizophyllum or Coprinus, whereas five could not be identified unequivocally. A rapid diagnostic overview is obtained with HaeIII and Hinf I digestion of the ITS region. Zusammenfassung: Elf klinische und veterinäre Stämme filamentöser Basidiomyzeten wurden untereinander und mit fünfzehn Referenzstämmen der Ordnungen Aphyllophorales und Agaricales verglichen. Angewandt wurden Restriktionsanalysen der SSU rDNA und der ITS 1 und 2 und Sequenzierung von Region V9‐ITS1. Sechs Stämme wurden als Repräsentanten der teleomorphen Gattungen Schizophyllum oder Coprinus identifiziert; die übrigen fünf konnten nicht mit Sicherheit bestimmt werden. Eine rasche diagnostische Übersicht wurde durch HaeIII und HinfI Verdauung der ITS Region erreicht.
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Samson, R. A., Yilmaz, N., Houbraken, J., Spierenburg, H., Seifert, K. A., Peterson, S. W., Varga, J., & Frisvad, J. C. (2011) · Studies in Mycology · doi:10.3114/sim.2011.70.04 · Cited by 435
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Metadata
- Authors
- de Hoog, G. S., & van den Ende, A. H. G. G.
- Venue
- Mycoses
- Publisher
- Wiley
- Published
- 1998-05
- Volume
- 41
- Issue
- 5-6
- Pages
- 183-189
- ISSN
- 0027-5557 (ISSN-L)
- 0933-7407
- 1439-0507
- OpenAlex
- W1974166687
- Cited by
- 574
- References
- 0
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- closed
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- de Hoog, G. S., & van den Ende, A. H. G. G. (1998). Molecular diagnostics of clinical strains of filamentous Basidiomycetes: Molekulare Diagnostik klinischer Stämme filamentöser Basidiomyzeten. Mycoses. https://doi.org/10.1111/j.1439-0507.1998.tb00321.x
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