Addition of DNase improves the in vitro activity of antifungal drugs against Candida albicans biofilms
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Summary: Cells within Candida albicans biofilms display decreased susceptibility to most clinically used antifungal agents. We recently demonstrated that extracellular DNA (eDNA) plays an important role in biofilm integrity, as a component of the biofilm matrix. This study aimed at gaining insights into the contributions of eDNA to C. albicans biofilms antifungal susceptibility by the investigation of the impact of the combined use of deoxyribonuclease I (DNase) and antifungals to treat biofilms. Candida albicans biofilms were formed using a simple and reproducible 96‐well plate‐based method. The activity of the combined use of 0.13 mg l−1 DNase and antifungals was estimated using the 2,3‐bis(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐5‐[(phenylamino)carbonyl]‐2 H‐tetrazolium hydroxide (XTT) reduction assay and total viable counts. Herein, we report the improved efficacy of amphotericin B when in combination with DNase against C. albicans biofilms, as assessed using XTT readings and viable counts. Furthermore, although DNase increased the efficacy of caspofungin in the reduction of mitochondrial activity, no changes were observed in terms of culturable cells. Deoxyribonuclease I did not affect biofilm cells susceptibility to fluconazole. This work suggests that agents that target processes affecting the biofilm structural integrity may have potential use as adjuvants of a catheter–lock therapy.
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In Candida species biofilms, carbohydrates, proteins, and nucleic acids form the extracellular matrix surrounding the cells.
“In Candida species biofilms, carbohydrates, proteins, and nucleic acids form the extracellular matrix that surround the cells in the biofilms [149,150,151,152,153,154].”
From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [153]
Treatment of biofilms with DNAse enhanced the activity of caspofungin and amphotericin B in disrupting mature C. albicans biofilms.
“For example, one study reported that treatment of biofilms with DNAse enhanced the activity of caspofungin and amphotericin B in disrupting mature C. albicans biofilms (116).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [116]
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References 26 · Cited by 174
- Authors
- Martins, M., Henriques, M., Lopez‐Ribot, J. L., & Oliveira, R.
- Affiliations
- Institute for Biotechnology and Bioengineering (PT)
- Corresponding author
- Oliveira, Rosário
- Venue
- Mycoses
- Publisher
- Wiley
- Published
- 2011-06-12
- Volume
- 55
- Issue
- 1
- Pages
- 80-85
- ISSN
- 0027-5557 (ISSN-L)
- 0933-7407
- 1439-0507
- PMC
- PMC3175262
- OpenAlex
- W1901946155
- Cited by
- 174
- References
- 26
- Citations by year
- 2026: 3 · 2025: 10 · 2024: 12 · 2023: 10 · 2022: 10 · 2021: 15 · 2020: 10 · 2019: 8 · 2018: 7 · 2017: 19 · 2016: 15 · 2015: 16 · 2014: 17 · 2013: 15 · 2012: 6
- FWCI
- 7.71
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Mycoses (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
- Funders
- National Institute of Allergy and Infectious Diseases (R21AI080930)
- National Institute of Dental and Craniofacial Research (R21DE017294)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Candida albicans, biofilm, DNase I, extracellular DNA, antifungal susceptibility, amphotericin B, caspofungin, fluconazole, biofilm matrix, catheter lock therapy
- MeSH terms
- Caspofungin, Amphotericin B, Antifungal Agents, Candida albicans, Deoxyribonucleases, Drug Combinations, Microbial Sensitivity Tests, Mitochondria, Tetrazolium Salts, Reproducibility of Results, Fluconazole, Biofilms, Echinocandins, Lipopeptides
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