MECHANISM OF THE FORMATION OF MEGAMITOCHONDRIA BY COPPER‐CHELATING AGENTS V. FURTHER STUDIES ON ISOLATED MEGAMITOCHONDRIA
Abstract
Effect of cuprizone has been studied on some biochemical properties of megamitochondria obtained from the mouse liver. (1) Contents of Ca2+, Mg2+ and Cu2+ in the blood or the liver homogenates were not altered by cuprizone intoxication, whereas those in liver mitochondria were significantly altered: after 3-4 days' intoxication, content of Ca2+ was decreased and was remarkably increased after 14-15 days' intoxication. Content of Mg2+ behaved contrarily. (2) Both cytochrome oxidase and ATPase activities were unchanged in the liver megamitochondria, but monoamine oxidase (MAO) activity was significantly decreased. Value of I50 (50% inhibition) for MAO was determined to be 0.33 mM using the control liver mitochondria. Cuprizone had almost no effect on MAO activity of kidney or heart mitochondria both in vivo and in vitro. (3) The amount of lysolecithin was increased in the liver megamitochondria. These results were discussed in the light of membrane fusion phenomenon which plays a key role in the mechanism of megamitochondrial formation.
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Cuprizone selectively affects oligodendrocytes.
“Nevertheless, a consensus seems to be that cuprizone selectively affects oligodendrocytes.”
From Strain differences in cuprizone induced demyelination (2017) · cites this paper as [21]
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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works
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Praet, J., Guglielmetti, C., Berneman, Z., Van der Linden, A., & Ponsaerts, P. (2014) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2014.10.004 · Cited by 435 · see citing works
Strain differences in cuprizone induced demyelination
Yu, Q., Hui, R., Park, J., Huang, Y., Kusnecov, A. W., Dreyfus, C. F., & Zhou, R. (2017) · Cell & Bioscience · doi:10.1186/s13578-017-0181-3 · Cited by 40 · see citing works
- Authors
- Wakabayashi, T., Asano, M., Ishikawa, K., & Kishimoto, H.
- Affiliations
- Nagoya City University (JP)
- Corresponding author
- Wakabayashi, Takashi
- ORCID
- Ishikawa, Kumiko
- Venue
- Acta Pathologica Japonica
- Publisher
- Wiley
- Published
- 1978-03
- Volume
- 28
- Issue
- 2
- Pages
- 215-223
- ISSN
- 0001-6632 (ISSN-L)
- OpenAlex
- W2081857795
- Cited by
- 14
- References
- 1
- Citations by year
- 2021: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2014: 1
- FWCI
- 0.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Pathologica Japonica (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- cuprizone, copper chelating agents, megamitochondria, liver mitochondria, monoamine oxidase, membrane fusion, lysophosphatidylcholine, cytochrome c oxidase, ATPase, mouse liver
- MeSH terms
- Adenosine Triphosphatases, Animals, Cuprizone, Cyclohexanes, Electron Transport Complex IV, Male, Metals, Mitochondria, Liver, Phospholipids, Mice, In Vitro Techniques
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