Matrix metalloproteinases MMP2 and MMP9 are upregulated by noradrenaline in the mouse neuroendocrine hypothalamus
Open accessAbstract
Magnocellular neurons of the hypothalamic supraoptic nuclei (SON) are involved in the synthesis and release of two major neuropeptides: oxytocin (OT) and arginine‐vassopressin (AVP). Neurochemical plasticity in this system is induced by physiological conditions such as lactation, parturition and dehydration, and may be accompanied by reversible structural plasticity affecting neurons, astrocytes and the extracellular matrix (ECM). The noradrenergic system plays a critical role in triggering this chemical plasticity associated with structural plasticity. Matrix metalloproteinases (MMPs) are good candidates for involvement in the ECM remodelling observed in structural plasticity. We investigated the possible regulation of the two gelatinases, MMP2 and MMP9, by noradrenaline (NA) in the mouse neuroendocrine hypothalamus. We looked for the presence, location and activity of MMP2 and MMP9 in the SON, using an ex vivo experimental model of mouse hypothalamic slices incubated for 4 h with 10−4 m NA. We showed that: (i) immunoreactivity for MMP2 and MMP9 was detected not only in AVP‐positive and OT‐positive magnocellular neurons, but also in astrocyte processes in control and NA‐treated slices; (ii) the number of MMP2‐ and MMP9‐positive cells increased after incubation with NA; (iii) MMP2 and MMP9 displayed markedly higher levels of gelatinolytic activity after NA treatment. These results suggest that both MMP2 and MMP9 are regulated by NA, and could therefore also be involved in structural plasticity within the SON.
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MMP9 is an important Aβ-degrading enzyme expressed on microglia and neurons.
“MMP9 is an important Aβ-degrading enzyme expressed on microglia (25) and neurons (26).”
From Heterozygous CX3CR1 Deficiency in Microglia Restores Neuronal β-Amyloid Clearance Pathways and Slows Progression of Alzheimer's Like-Disease in PS1-APP Mice (2019) · cites this paper as [26]
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- Hindmarch, C., Yao, S., Geighton, G., Paton, J. & Murphy, D. (2006) A comprehensive description of the transcriptome of the hypothalamoneurohy- pophyseal system in euhydrated and dehydrated rats. Proc. Natl Acad. Sci. USA, 103, 1609-1614.
- Piet, R., Vargova, L., Sykova, E., Poulain, D.A. & Oliet, S.H. (2004) Physiological contribution of the astrocytic environment of neurons to intersynaptic crosstalk. Proc. Natl Acad. Sci. USA, 101, 2151-2155.
- Rivera, S. & Khrestchatisky, M. (1999) Matrix metalloproteinases and tissue inhibitors of metalloproteinases in neuronal plasticity and pathology. In Baudry. M., Thomsom, R.F. & Davis, J.L. (Eds), Advances in Synaptic Plasticity. MIT Press, Cambridge, Massachusetts, pp. 53-86.
- Sofroniew, M.V. (1985) Vasopressin, oxytocin and their related neurophysins. In Bjorklund, A. & Hokfelt, T. (Eds), Handbook of Chemical Neuroanatomy. Elsevier, Amsterdam, pp. 93-152.
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Hickman, S. E., Allison, E. K., Coleman, U., Kingery-Gallagher, N. D., & El Khoury, J. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.02780 · Cited by 80 · see citing works
- Authors
- Maolood, N., Hardin‐Pouzet, H., & Grange‐Messent, V.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Grange‐Messent, V.
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2008-03
- Volume
- 27
- Issue
- 5
- Pages
- 1143-1152
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2130859238
- Cited by
- 33
- References
- 0
- Citations by year
- 2026: 2 · 2025: 2 · 2023: 3 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 1
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- matrix metalloproteinases, MMP-2, MMP-9, norepinephrine, supraoptic nucleus, magnocellular neurons, oxytocin, vasopressin, extracellular matrix remodeling, structural plasticity, astrocytes, gelatinase activity
- MeSH terms
- Animals, Enzyme Induction, Hypothalamus, Male, Mice, Inbred C3H, Neurosecretory Systems, Norepinephrine, Up-Regulation, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Mice
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