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Cited by 262

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass

Open access
Andrianov, Vyacheslav, Borisjuk, Nikolai, Pogrebnyak, Natalia, Brinker, Anita, Dixon, Joseph, Spitsin, Sergei, Flynn, John, Matyszczuk, Paulina, Andryszak, Karolina, Laurelli, Marilyn, Golovkin, Maxim, Koprowski, Hilary·2010·Plant Biotechnology Journal
DOI 10.1111/j.1467-7652.2009.00458.x
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Abstract

Summary: When grown for energy production instead for smoking, tobacco can generate a large amount of inexpensive biomass more efficiently than almost any other agricultural crop. Tobacco possesses potent oil biosynthesis machinery and can accumulate up to 40% of seed weight in oil. In this work, we explored two metabolic engineering approaches to enhance the oil content in tobacco green tissues for potential biofuel production. First, an Arabidopsis thaliana gene diacylglycerol acyltransferase (DGAT) coding for a key enzyme in triacylglycerol (TAG) biosynthesis, was expressed in tobacco under the control of a strong ribulose‐biphosphate carboxylase small subunit promoter. This modification led to up to a 20‐fold increase in TAG accumulation in tobacco leaves and translated into an overall of about a twofold increase in extracted fatty acids (FA) up to 5.8% of dry biomass in Nicotiana tabacum cv Wisconsin, and up to 6% in high‐sugar tobacco variety NC‐55. Modified tobacco plants also contained elevated amounts of phospholipids. This increase in lipids was accompanied by a shift in the FA composition favourable for their utilization as biodiesel. Second, we expressed in tobacco Arabidopsis gene LEAFY COTYLEDON 2 (LEC2), a master regulator of seed maturation and seed oil storage under the control of an inducible Alc promoter. Stimulation of LEC2 expression in mature tobacco plants by acetaldehyde led to the accumulation of up to 6.8% per dry weight of total extracted FA. The obtained data reveal the potential of metabolically modified plant biomass for the production of biofuel.

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Metadata

Authors
Andrianov, V., Borisjuk, N., Pogrebnyak, N., Brinker, A., Dixon, J., Spitsin, S., Flynn, J., Matyszczuk, P., Andryszak, K., Laurelli, M., Golovkin, M., & Koprowski, H.
Venue
Plant Biotechnology Journal
Publisher
Wiley
Published
2010-03-02
Volume
8
Issue
3
Pages
277-287
ISSN
  • 1467-7644 (ISSN-L)
  • 1467-7652
DOI
10.1111/j.1467-7652.2009.00458.x
OpenAlex
W2059230904
Cited by
262
References
34
FWCI
5.11
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass. https://app.cochat.ai/papers/10.1111/j.1467-7652.2009.00458.x
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Andrianov, V., Borisjuk, N., Pogrebnyak, N., Brinker, A., Dixon, J., Spitsin, S., Flynn, J., Matyszczuk, P., Andryszak, K., Laurelli, M., Golovkin, M., & Koprowski, H. (2010). Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass. Plant Biotechnology Journal. https://doi.org/10.1111/j.1467-7652.2009.00458.x

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.