Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae
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Rapid, accurate and inexpensive diagnosis of bacterial meningitis is critical for patient management. This study describes the development and evaluation of a multiplex PCR assay for the detection of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae type b, which globally account for 90% of cases of bacterial meningitis. The single-tube assay, based on the ctrA, ply and bex targets, respectively, enabled detection of 5-10 pg DNA. When the assay was tested with clinical samples (n = 425), its sensitivity for the three targets was 93.9%, 92.3% and 88%, respectively, while the overall specificity and positive predictive value of the assay was 100%. The negative predictive value was 99.1-99.5%. The methodology permits rapid and accurate detection of the three main pathogens that cause bacterial meningitis.
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Other studies looking at single organism-specific PCR or multiplex assays for a variety of organisms, such as H. influenzae, S. pneumoniae, and N. meningitidis, have demonstrated increased sensitivity compared to conventional methods.
“Other studies looking at single organism-specific PCR or multiplex assays for a variety of organisms, such as H. influenzae, S. pneumoniae, and N. meningitidis, have demonstrated increased sensitivity compared to conventional methods (28–30).”
From Multicenter Evaluation of BioFire FilmArray Meningitis/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens (2016) · cites this paper as [29]
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References 30 · Cited by 106
- Authors
- Tzanakaki, G., Tsopanomichalou, M., Kesanopoulos, K., Matzourani, R., Sioumala, M., Tabaki, A., & Kremastinou, J.
- Affiliations
- National School of Public Health (GR)
- Corresponding author
- Tzanakaki, G.
- Venue
- Clinical Microbiology and Infection
- Publisher
- Elsevier BV
- Published
- 2005-05
- Volume
- 11
- Issue
- 5
- Pages
- 386-390
- ISSN
- 1198-743X (ISSN-L)
- OpenAlex
- W1554665084
- Cited by
- 106
- References
- 30
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 7 · 2022: 7 · 2021: 4 · 2020: 2 · 2019: 6 · 2018: 3 · 2017: 7 · 2016: 12 · 2015: 7 · 2014: 5 · 2013: 7 · 2012: 3
- FWCI
- 3.90
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Clinical Microbiology and Infection (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Neonatal and Maternal Infections
- Keywords
- bacterial meningitis, Neisseria meningitidis, Haemophilus influenzae type b, Streptococcus pneumoniae, multiplex PCR, molecular diagnosis, sensitivity and specificity
- MeSH terms
- Bacterial Proteins, Diagnosis, Differential, DNA-Binding Proteins, DNA, Bacterial, Humans, Meningitis, Haemophilus, Meningitis, Meningococcal, Meningitis, Pneumococcal, Neisseria meningitidis, Pneumococcal Infections, Sensitivity and Specificity, Streptococcus pneumoniae, Streptolysins, Transcription Factors, Polymerase Chain Reaction, Meningitis, Bacterial, Haemophilus influenzae type b, Membrane Transport Proteins
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