Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum

Koek, A.G.; Bruisten, S.M.; Dierdorp, M.; van Dam, A.P.; Templeton, K.

doi:10.1111/j.1469-0691.2006.01566.x

Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum

A real-time PCR assay with a Taqman probe was developed that targeted the polA gene of Treponema pallidum. The test was validated using an analytical panel (n = 140) and a clinical panel of genital samples (n = 112) from patients attending a sexually transmitted infections clinic. High sensitivit…