Silencing two herbivory‐activated MAP kinases, SIPK and WIPK, does not increase Nicotiana attenuata's susceptibility to herbivores in the glasshouse and in nature
Open accessAbstract
Salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) are activated by Manduca sexta attack and elicitors to mediate defense signaling in Nicotiana attenuata. Here, the ecological consequences of SIPK and WIPK silencing for N. attenuata's resistance to M. sexta and its other native herbivores were analyzed. Stably transformed plants with reduced expression of NaSIPK (irNaSIPK) and NaWIPK(irNaWIPK) were generated and characterized in field and glasshouse experiments. Both irNaSIPK and irNaWIPK plants had reduced direct and indirect defenses but were not particularly susceptible in nature. In the glasshouse, M. sexta larvae consumed less and gained the same mass on irNaSIPK and irNaWIPK as on wild-type (WT) plants. Green leaf volatile (GLV) emission was highly attenuated in irNaSIPK and irNaWIPK plants, and complementation with synthetic GLVs increased M. sexta performance. To test the hypothesis that reduced GLV emissions account for the lack of herbivory phenotype, GLV emissions were attenuated by silencing NaHPL in jasmonate-deficient plants (asNaLOX3), which are highly susceptible to herbivores. Reducing GLV emissions in asNaLOX3 plants 'rescued' these plants from being heavily damaged by M. sexta. GLV emissions in irNaSIPK and irNaWIPK plants may compensate for the impaired defenses of NaSIPK- and NaWIPK-silenced plants in nature by reducing their apparency to herbivores.
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Several other herbivores gain more weight in the presence of plant secondary metabolites.
“Several other herbivores also gain more weight in the presence of plant secondary metabolites (Meldau et al., 2009;Richards et al., 2012;Marti et al., 2013;Veyrat et al., 2016;Wetzel et al., 2016), and it is conceivable that some of these effects may be due to the capacity of the herbivores to metabolize these compounds.”
From Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy (2020) · cites this paper as Meldau et al., 2009
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Using ‘mute’ plants to translate volatile signals
Paschold, A., Halitschke, R., & Baldwin, I. T. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2005.02623.x · Cited by 151 · see citing works
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Erb, M., & Kliebenstein, D. J. (2020) · Plant Physiology · doi:10.1104/pp.20.00433 · Cited by 1,333 · see citing works
- Authors
- Meldau, S., Wu, J., & Baldwin, I. T.
- Affiliations
- Max Planck Institute for Chemical Ecology (DE)
- Corresponding author
- Baldwin, Ian T.
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2008-12-03
- Volume
- 181
- Issue
- 1
- Pages
- 161-173
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2103024721
- Cited by
- 90
- References
- 1
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 6 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 5 · 2013: 9 · 2012: 10
- FWCI
- 11.68
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- New Phytologist (publishedVersion, other-oa)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Agriculture
- Brigham Young University
- Max-Planck-Gesellschaft
- Animal and Plant Health Inspection Service
- Topics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Keywords
- SIPK, mitogen-activated protein kinase, Manduca sexta, herbivory, plant defense signaling, green leaf volatiles, jasmonates, indirect plant defense, herbivore susceptibility, Nicotiana attenuata, gene silencing
- MeSH terms
- Animals, Cyclopentanes, Feeding Behavior, Nicotine, Oils, Volatile, Plant Proteins, Nicotiana, Gene Expression, Manduca, Gene Expression Regulation, Plant, Gene Silencing, Mitogen-Activated Protein Kinases, Plants, Genetically Modified, Oxylipins
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