The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
454 Pyrosequencing and Sanger sequencing of tropical mycorrhizal fungi provide similar results but reveal substantial methodological biases
Open accessAbstract
• Compared with Sanger sequencing-based methods, pyrosequencing provides orders of magnitude more data on the diversity of organisms in their natural habitat, but its technological biases and relative accuracy remain poorly understood. • This study compares the performance of pyrosequencing and traditional sequencing for species' recovery of ectomycorrhizal fungi on root tips in a Cameroonian rain forest and addresses biases related to multi-template PCR and pyrosequencing analyses. • Pyrosequencing and the traditional method yielded qualitatively similar results, but there were slight, but significant, differences that affected the taxonomic view of the fungal community. We found that most pyrosequencing singletons were artifactual and contained a strongly elevated proportion of insertions compared with natural intra- and interspecific variation. The alternative primers, DNA extraction methods and PCR replicates strongly influenced the richness and community composition as recovered by pyrosequencing. • Pyrosequencing offers a powerful alternative for the identification of ectomycorrhizal fungi in pooled root samples, but requires careful selection of molecular tools. A well-populated backbone database facilitates the detection of biological and technical artifacts. The pyrosequencing pipeline is available at http://unite.ut.ee/454pipeline.tgz.
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Fungal community analysis by high‐throughput sequencing of amplified markers – a user's guide
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Comparison and Validation of Some ITS Primer Pairs Useful for Fungal Metabarcoding Studies
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Metadata
- Authors
- Tedersoo, L., Nilsson, R. H., Abarenkov, K., Jairus, T., Sadam, A., Saar, I., Bahram, M., Bechem, E., Chuyong, G., & Kõljalg, U.
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2010-09-02
- Volume
- 188
- Issue
- 1
- Pages
- 291-301
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2156417649
- Cited by
- 549
- References
- 48
- FWCI
- 54.21
- Type
- journal article
- Language
- en
- Open access
- bronze
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