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A Semiautomated Analysis Method for Catecholamines, Indoleamines, and Some Prominent Metabolites in Microdissected Regions of the Nervous System: An Isocratic HPLC Technique Employing Coulometric Detection and Minimal Sample Preparation
Abstract
The application of a commercially available coulometric electrochemical detector to the automated HPLC analysis of some monoamines and their metabolites in microdissected areas of the rat nervous system is described. Apart from the stability and high sensitivity of the system, other appealing features of the technique are the facile sample preparation and long‐term sample storage characteristics which show minimal analyte degradation. Basal values of some regional monoamine and metabolite concentration are listed together with a brief appendix that serves as a user's guide to the operation and maintenance of the detection system.
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Citations
Cited by
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Dalla, C., Antoniou, K., Drossopoulou, G., Xagoraris, M., Kokras, N., Sfikakis, A., & Papadopoulou-Daifoti, Z. (2005) · Neuroscience · doi:10.1016/j.neuroscience.2005.06.068 · Cited by 340
McCann, U. D., Ridenour, A., Shaham, Y., & Ricaurte, G. A. (1994) · Neuropsychopharmacology · doi:10.1038/npp.1994.15 · Cited by 332
Petzinger, G. M., Walsh, J. P., Akopian, G., Hogg, E., Abernathy, A., Arevalo, P., Turnquist, P., Vučković, M., Fisher, B. E., Togasaki, D. M., et al. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.1069-07.2007 · Cited by 329
McCann, U. D., Mertl, M., Eligulashvili, V., & Ricaurte, G. A. (1999) · Psychopharmacology · doi:10.1007/s002130050967 · Cited by 300
Matsuoka, Y., Vila, M., Lincoln, S., McCormack, A., Picciano, M., LaFrancois, J., Yu, X., Dickson, D., Langston, W. J., McGowan, E., et al. (2001) · Neurobiology of Disease · doi:10.1006/nbdi.2001.0392 · Cited by 281
Metadata
- Authors
- Kilpatrick, I. C., Jones, M. W., & Phillipson, O. T.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1986-06
- Volume
- 46
- Issue
- 6
- Pages
- 1865-1876
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W1978513925
- Cited by
- 195
- References
- 0
- FWCI
- 13.04
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A Semiautomated Analysis Method for Catecholamines, Indoleamines, and Some Prominent Metabolites in Microdissected Regions of the Nervous System: An Isocratic HPLC Technique Employing Coulometric Detection and Minimal Sample Preparation. https://app.cochat.ai/papers/10.1111/j.1471-4159.1986.tb08506.x
- The paper
- Kilpatrick, I. C., Jones, M. W., & Phillipson, O. T. (1986). A Semiautomated Analysis Method for Catecholamines, Indoleamines, and Some Prominent Metabolites in Microdissected Regions of the Nervous System: An Isocratic HPLC Technique Employing Coulometric Detection and Minimal Sample Preparation. Journal of Neurochemistry. https://doi.org/10.1111/j.1471-4159.1986.tb08506.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.