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An inexpensive, automation‐friendly protocol for recovering high‐quality DNA
Abstract
Although commercial kits are available for automated DNA extraction, ‘artisanal’ protocols are not. In this study, we present a silica‐based method that is sensitive, inexpensive and compliant with automation. The effectiveness of this protocol has now been tested on more than 5000 animal specimens with highly positive results.
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Cited by
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Ward, R. D., Hanner, R., & Hebert, P. D. N. (2009) · Journal of Fish Biology · doi:10.1111/j.1095-8649.2008.02080.x · Cited by 964
Self-citationOpportunities for biodiversity gains under the world’s largest reforestation programme
Hua, F., Wang, X., Zheng, X., Fisher, B., Wang, L., Zhu, J., Tang, Y., Yu, D. W., & Wilcove, D. S. (2016) · Nature Communications · doi:10.1038/ncomms12717 · Cited by 416
deWaard, J. R., Ivanova, N. V., Hajibabaei, M., & Hebert, P. D. N. (2008) · Methods in Molecular Biology · doi:10.1007/978-1-59745-548-0_15 · Cited by 389
Self-citationEnvironmental metabarcodes for insects: in silicoPCR reveals potential for taxonomic bias
Clarke, L. J., Soubrier, J., Weyrich, L. S., & Cooper, A. (2014) · Molecular Ecology Resources · doi:10.1111/1755-0998.12265 · Cited by 351
Can DNA barcoding accurately discriminate megadiverse Neotropical freshwater fish fauna?
Pereira, L. H., Hanner, R., Foresti, F., & Oliveira, C. (2013) · BMC Genetics · doi:10.1186/1471-2156-14-20 · Cited by 329
Metadata
- Authors
- IVANOVA, N. V., DEWAARD, J. R., & HEBERT, P. D. N.
- Venue
- Molecular Ecology Notes
- Publisher
- Wiley
- Published
- 2006-07-07
- Volume
- 6
- Issue
- 4
- Pages
- 998-1002
- ISSN
- 1471-8278 (ISSN-L)
- 1471-8286
- OpenAlex
- W1605875024
- Cited by
- 1,475
- References
- 0
- FWCI
- 8.60
- Type
- journal article
- Language
- en
- Open access
- closed
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- IVANOVA, N. V., DEWAARD, J. R., & HEBERT, P. D. N. (2006). An inexpensive, automation‐friendly protocol for recovering high‐quality DNA. Molecular Ecology Notes. https://doi.org/10.1111/j.1471-8286.2006.01428.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
