The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
A minimalist barcode can identify a specimen whose DNA is degraded
Abstract
A DNA barcode based on 650 bp of mitochondrial gene cytochrome c oxidase I is proving to be highly functional in species identification for various animal groups. However, DNA degradation complicates the recovery of a full‐length barcode from many museum specimens. Here we explore the use of shorter barcode sequences for identification of such specimens. We recovered short sequences — i.e. ∼100 bp — with a single PCR pass from more than 90% of the specimens in assemblages of moth and wasp museum specimens from which full barcode recovery was only 50%, and the latter were usually less than 8 years old. Short barcodes were effective in identifying specimens, confirming their utility in circumstances where full barcodes are too expensive to obtain and the identification comparisons are within a confined taxonomic group.
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Cites
Kimura, M. (1980) · Journal of Molecular Evolution · doi:10.1007/bf01731581 · Cited by 29,778
DNA barcoding: error rates based on comprehensive sampling
Meyer, C. P., & Paulay, G. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030422 · Cited by 1,957
Cited by
Hajibabaei, M., Shokralla, S., Zhou, X., Singer, G. A. C., & Baird, D. J. (2011) · PLoS ONE · doi:10.1371/journal.pone.0017497 · Cited by 584
Self-citationCarew, M. E., Pettigrove, V. J., Metzeling, L., & Hoffmann, A. A. (2013) · Frontiers in Zoology · doi:10.1186/1742-9994-10-45 · Cited by 243
Armani, A., Tinacci, L., Giusti, A., Castigliego, L., Gianfaldoni, D., & Guidi, A. (2013) · Food Research International · doi:10.1016/j.foodres.2013.10.003 · Cited by 66
Metadata
- Authors
- HAJIBABAEI, M., SMITH, M. A., JANZEN, D. H., RODRIGUEZ, J. J., WHITFIELD, J. B., & HEBERT, P. D. N.
- Venue
- Molecular Ecology Notes
- Publisher
- Wiley
- Published
- 2006-07-07
- Volume
- 6
- Issue
- 4
- Pages
- 959-964
- ISSN
- 1471-8278 (ISSN-L)
- 1471-8286
- OpenAlex
- W2170596217
- Cited by
- 559
- References
- 2
- FWCI
- 17.89
- Type
- journal article
- Language
- en
- Open access
- closed
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- HAJIBABAEI, M., SMITH, M. A., JANZEN, D. H., RODRIGUEZ, J. J., WHITFIELD, J. B., & HEBERT, P. D. N. (2006). A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes. https://doi.org/10.1111/j.1471-8286.2006.01470.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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