The DOI matches its OpenAlex record; 54 of the 54 references it cites were found in a registry or an index.
Abundance and spatial organization of Gram-negative sulfate-reducing bacteria in activated sludge investigated by in situ probing with specific 16S rRNA targeted oligonucleotides
Abstract
A comprehensive panel of ten 16S rRNA targeted oligonucleotides specific for mesophilic sulfate-reducing bacteria (SRB) within the δ-subclass of Proteobacteria was developed as a diagnostic tool and evaluated for its specificity and in situ applicability. Five probes (DSD131, DSBO224, DSV407, DSR651, DSS658) are specific on genus level and five probes identify distinct phylogenetic subbranches within the families Desulfobacteriaceae (DSMA488, DSB985) and Desulfovibrionaceae (DSV214, DSV698, DSV1292). All oligonucleotides were checked for their specificity by computer aided comparative sequence analysis. For in situ application optimal stringency conditions were adjusted for each fluorescence-labelled probe performing whole cell hybridizations using target and non-target organisms. Activated sludge flocs from different stages within the aeration tank of a large municipal wastewater treatment plant were examined by in situ hybridizations with the indocarbocyanine- (Cy3-) labelled SRB-specific probes. The relative abundance and the spatial organization of single SRB were monitored with epifluorescence and confocal laser scanning microscopy. Individual sulfate-reducing cells could be visualized and the number of cells ranged from 0.5 to 8% of the total cell counts within all stages of the activated sludge process and the final clarifier. Cells yielding strong fluorescence signals after hybridization with the newly developed probes were not restricted to anoxic and anaerobic compartments, but were also clearly detectable in the aeration zones of the treatment plant.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
(1987) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040454 · Cited by 59,715
Phylogenetic identification and in situ detection of individual microbial cells without cultivation
Amann, R., Ludwig, W., & SCHLEIFER, K. H. (1995) · Microbiological Reviews · doi:10.1128/mmbr.59.1.143-169.1995 · Cited by 9,093
Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R., & Stahl, D. A. (1990) · Applied and Environmental Microbiology · doi:10.1128/aem.56.6.1919-1925.1990 · Cited by 4,021
Manz, W., Amann, R., Ludwig, W., Wagner, M., & Schleifer, K.-H. (1992) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(11)80121-9 · Cited by 2,043
Self-citationGene organization and primary structure of a ribosomal RNA operon from Escherichia coli
Brosius, J., Dull, T. J., Sleeter, D. D., & Noller, H. F. (1981) · Journal of Molecular Biology · doi:10.1016/0022-2836(81)90508-8 · Cited by 1,707
Staley, J. T., & Konopka, A. (1985) · Annual Review of Microbiology · doi:10.1146/annurev.mi.39.100185.001541 · Cited by 1,570
Gram-Negative Mesophilic Sulfate-Reducing Bacteria
Widdel, F., & Bak, F. (1992) · The Prokaryotes · doi:10.1007/978-1-4757-2191-1_21 · Cited by 1,551
Schaechter, M., MaalOe, O., & Kjeldgaard, N. O. (1958) · Journal of General Microbiology · doi:10.1099/00221287-19-3-592 · Cited by 1,435
Lovley, D. R., Giovannoni, S. J., White, D. C., Champine, J. E., Phillips, E. J. P., Gorby, Y. A., & Goodwin, S. (1993) · Archives of Microbiology · doi:10.1007/bf00290916 · Cited by 983
Microbiology and ecology of sulfate-and sulfur-reducing bacteria
Widdel, F. (1988) · Medical Entomology and Zoology · Cited by 799
Cited by
Duncan, S. H., Belenguer, A., Holtrop, G., Johnstone, A. M., Flint, H. J., & Lobley, G. E. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02340-06 · Cited by 941
Artificial electron acceptors decouple archaeal methane oxidation from sulfate reduction
Scheller, S., Yu, H., Chadwick, G. L., McGlynn, S. E., & Orphan, V. J. (2016) · Science · doi:10.1126/science.aad7154 · Cited by 441
Lösekann, T., Knittel, K., Nadalig, T., Fuchs, B., Niemann, H., Boetius, A., & Amann, R. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.00016-07 · Cited by 391
Swidsinski, A. (2005) · World Journal of Gastroenterology · doi:10.3748/wjg.v11.i8.1131 · Cited by 314
Hoffmann, F., Larsen, O., Thiel, V., Rapp, H. T., Pape, T., Michaelis, W., & Reitner, J. (2005) · Geomicrobiology Journal · doi:10.1080/01490450590922505 · Cited by 233
Metadata
- Authors
- Manz, W., Eisenbrecher, M., Neu, T. R., & Szewzyk, U.
- Venue
- FEMS Microbiology Ecology
- Publisher
- Oxford University Press
- Published
- 1998-01-01
- Volume
- 25
- Issue
- 1
- Pages
- 43-61
- ISSN
- 0168-6496 (ISSN-L)
- OpenAlex
- W1987576241
- Cited by
- 277
- References
- 54
- FWCI
- 14.87
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Abundance and spatial organization of Gram-negative sulfate-reducing bacteria in activated sludge investigated by in situ probing with specific 16S rRNA targeted oligonucleotides. https://app.cochat.ai/papers/10.1016/s0168-6496(97)00082-2
- The paper
- Manz, W., Eisenbrecher, M., Neu, T. R., & Szewzyk, U. (1998). Abundance and spatial organization of Gram-negative sulfate-reducing bacteria in activated sludge investigated by in situ probing with specific 16S rRNA targeted oligonucleotides. FEMS Microbiology Ecology. https://doi.org/10.1016/s0168-6496(97)00082-2
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
