The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Improved PCR primers for the detection and identification of arbuscular mycorrhizal fungi: PCR primers for arbuscular mycorrhizal fungi
Abstract
A set of PCR primers that should amplify all subgroups of arbuscular mycorrhizal fungi (AMF, Glomeromycota), but exclude sequences from other organisms, was designed to facilitate rapid detection and identification directly from field-grown plant roots. The small subunit rRNA gene was targeted for the new primers (AML1 and AML2) because phylogenetic relationships among the Glomeromycota are well understood for this gene. Sequence comparisons indicate that the new primers should amplify all published AMF sequences except those from Archaeospora trappei. The specificity of the new primers was tested using 23 different AMF spore morphotypes from trap cultures and Miscanthus sinensis, Glycine max and Panax ginseng roots sampled from the field. Non-AMF DNA of 14 plants, 14 Basidiomycota and 18 Ascomycota was also tested as negative controls. Sequences amplified from roots using the new primers were compared with those obtained using the established NS31 and AM1 primer combination. The new primers have much better specificity and coverage of all known AMF groups.
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Fungal community analysis by high‐throughput sequencing of amplified markers – a user's guide
Lindahl, B. D., Nilsson, R. H., Tedersoo, L., Abarenkov, K., Carlsen, T., Kjøller, R., Kõljalg, U., Pennanen, T., Rosendahl, S., Stenlid, J., et al. (2013) · New Phytologist · doi:10.1111/nph.12243 · Cited by 892
Krüger, M., Stockinger, H., Krüger, C., & Schüßler, A. (2009) · New Phytologist · doi:10.1111/j.1469-8137.2009.02835.x · Cited by 389
PacBio metabarcoding of Fungi and other eukaryotes: errors, biases and perspectives
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Routes to roots: direct evidence of water transport by arbuscular mycorrhizal fungi to host plants
Kakouridis, A., Hagen, J. A., Kan, M. P., Mambelli, S., Feldman, L. J., Herman, D. J., Weber, P. K., Pett‐Ridge, J., & Firestone, M. K. (2022) · New Phytologist · doi:10.1111/nph.18281 · Cited by 292
Zheng, Y., Chen, L., Luo, C.-Y., Zhang, Z.-H., Wang, S.-P., & Guo, L.-D. (2016) · Microbial Ecology · doi:10.1007/s00248-016-0817-6 · Cited by 36
Metadata
- Authors
- Lee, J., Lee, S., & Young, J. P. W.
- Venue
- FEMS Microbiology Ecology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-08
- Volume
- 65
- Issue
- 2
- Pages
- 339-349
- ISSN
- 0168-6496 (ISSN-L)
- 1574-6941
- OpenAlex
- W1789028138
- Cited by
- 871
- References
- 28
- FWCI
- 16.58
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lee, J., Lee, S., & Young, J. P. W. (2008). Improved PCR primers for the detection and identification of arbuscular mycorrhizal fungi: PCR primers for arbuscular mycorrhizal fungi. FEMS Microbiology Ecology. https://doi.org/10.1111/j.1574-6941.2008.00531.x
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