Molecular profiling and identification of methanogenic archaeal species from rabbit caecum: New methanogens from rabbit caecum
Open accessAbstract
During a comparison of 16S rDNA PCR-denaturant gradient gel electrophoresis (DGGE) profiles of methanogenic archaea from rumen fluid, rabbit caecum and pig feces, a unique band common to all rabbit caecum samples was observed. DGGE profiling also showed that the methanogen community from the New Zealand White adult rabbits is different and less complex than the methanogen communities from the rumen and pig feces. Small subunit ribosomal gene sequences of methanogenic archaea were subsequently retrieved from the constructed rabbit caecum 16S rDNA gene library. Results of the phylogenetic analysis indicated that rabbit caecum is inhabited by members of the genus Methanobrevibacter and is possibly one-species dominated, because all the retrieved sequences exhibited similarity values of 99% or higher. This species may well be a novel species of the genus Methanobrevibacter. It belongs to a distinct phylogenetic group containing Methanobrevibacter woesei, Methanobrevibacter thaueri and Methanobrevibacter gottschalkii strains isolated from animal feces, and Methanobrevibacter smithii from the predominating methanogen population of the human large bowel.
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The genus Methanobrevibacter is the most ubiquitous and predominant hydrogenotrophic methanogen in the foregut and hindgut of herbivores.
“The genus Methanobrevibacter is the most ubiquitous and predominant hydrogenotrophic methanogens found in the foregut and hindgut of herbivores (Kušar and Avguštin, 2010; St-Pierre and Wright, 2013) although several species of Methanomassiliicoccales can use methyl substrates (Poulsen et al., 2013).”
From Comparative Analysis of the Microbiota Between Sheep Rumen and Rabbit Cecum Provides New Insight Into Their Differential Methane Production (2018) · cites this paper as Kušar and Avguštin, 2010
Only one study has analyzed the archaeal microbiota in the cecum of rabbits, and M. woesei was represented by more cloned 16S rRNA gene sequences than other species.
“Based on a literature search of the Pubmed, only one study has analyzed the archaeal microbiota in the cecum of rabbits, and M. woesei was represented by more cloned 16S rRNA gene sequences than other species (Kušar and Avguštin, 2010).”
From Comparative Analysis of the Microbiota Between Sheep Rumen and Rabbit Cecum Provides New Insight Into Their Differential Methane Production (2018) · cites this paper as Kušar and Avguštin, 2010
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Mi, L., Yang, B., Hu, X., Luo, Y., Liu, J., Yu, Z., & Wang, J. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00575 · Cited by 50 · see citing works
- Authors
- Kušar, D., & Avguštin, G.
- Affiliations
- University of Ljubljana (SI)
- Corresponding author
- Avguštin, Gorazd
- Venue
- FEMS Microbiology Ecology
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-10-15
- Volume
- 74
- Issue
- 3
- Pages
- 623-630
- ISSN
- 0168-6496 (ISSN-L)
- OpenAlex
- W1753469818
- Cited by
- 48
- References
- 0
- Citations by year
- 2026: 2 · 2024: 4 · 2023: 1 · 2022: 2 · 2021: 6 · 2020: 2 · 2019: 8 · 2018: 4 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 4 · 2012: 4
- FWCI
- 7.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Ecology (publishedVersion)
- PubMed (publishedVersion)
- Repository of the University of Ljubljana (University of Ljubljana) (publishedVersion, other-oa)
- Funders
- Javna Agencija za Raziskovalno Dejavnost RS
- Topics
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Keywords
- methanogenic archaea, Methanobrevibacter, 16S rRNA gene, small subunit rRNA, rumen, pig feces, new species, phylogenetic analysis, molecular profiling, denaturing gradient gel electrophoresis
- MeSH terms
- Animals, Cecum, Phylogeny, Rabbits, RNA, Ribosomal, 16S, Sequence Analysis, DNA, DNA, Archaeal, Methanobrevibacter, Denaturing Gradient Gel Electrophoresis
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