The DOI matches its Crossref record; 19 of the 24 references it cites were found in a registry or an index.
Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation
Open accessAbstract
A method is presented for the introduction of plasmids into Clostridium acetobutylicum ATCC 8052 by electroporation. A plasmid shuttle vector, pMTL500E, which contains the erythromycin resistance gene and replication machinery of plasmid pAMβ1, was constructed and introduced into C. acetobutylicum by electroporation. The vector was then used to introduce a 2.2 kb ClaI/SphI chromosomal fragment from C. pasteurianum into a leucine requiring mutant of C. acetobutylicum, SBA9, where complementation of auxotrophy was observed. Plasmid DNA indistinguishable from that introduced, on the basis of agarose gel electrophoresis, was observed in transformants containing either plasmid.
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“Some of the well-studied, non-acetogenic clostridia, such as the ABE model organisms C. acetobutylicum (Mermelstein et al., 1992) and C. beijerinckii (Oultram et al., 1988), and important pathogens, such as C. difficile (Ackermann et al., 2001) and C. botulinum (Zhou and Johnson, 1993), have transformation protocols that were established as early as 1988.”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Oultram et al., 1988
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Metadata
- Authors
- Oultram, J. D., Loughlin, M., Swinfield, T.-J., Brehm, J. K., Thompson, D. E., & Minton, N. P.
- ORCID
- Minton, N.P.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1988-11
- Volume
- 56
- Issue
- 1
- Pages
- 83-88
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W1979262511
- Cited by
- 133
- References
- 24
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2019: 2 · 2017: 3 · 2016: 8 · 2015: 5 · 2014: 7 · 2013: 3 · 2012: 4
- FWCI
- 3.55
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Letters (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Clostridium acetobutylicum, electroporation, plasmid transformation, shuttle vector, erythromycin resistance, leucine auxotrophy
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