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The importance of PHB-synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus
Abstract
Alcaligenes eutrophus can accumulate poly-3-hydroxybutyrate (PHB) or polyhydroxyalkanoate (PHA) containing only 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV) units. Granule-associated PHB-synthase was active with d(−)-3-hydroxybutyryl-CoA and d(−)-3-hydroxyvaleryl-CoA of the range of d(−)- and l(+)-3-hydroxyacyl-CoA substrates tested (C4–C10). In carbon-limited cultures, PHB-synthase was predominantly soluble, becoming granule-associated on transition to nitrogen limitation. Granule-associated PHB-synthase increased in activity at least up to pH 10.0 and Km values of 0.68 mM and 1.63 mM were determined for the C4 and C5 substrates, respectively, at pH 8.5. The soluble PHB-synthase, which was unstable, showed equal activity in the range pH 8.0–10.0, had a Km value for d(−)-3-hydroxybutyryl-CoA of 0.72 mM and an Mr of 160,000. PHB does not measurably turn over under steady-state polymer-accumulating conditions.
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Citations
Cites
Enzymatic Synthesis of Poly-β-Hydroxybutyric Acid in Bacteria
MERRICK, J. M., & DOUDOROFF, M. (1961) · Nature · doi:10.1038/189890a0 · Cited by 100
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Lenz, R. W., & Marchessault, R. H. (2005) · Biomacromolecules · doi:10.1021/bm049700c · Cited by 780
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Poly(hydroxyalkanoates): A Fifth Class of Physiologically Important Organic Biopolymers?
Müller, H., & Seebach, D. (1993) · Angewandte Chemie International Edition in English · doi:10.1002/anie.199304771 · Cited by 363
Metadata
- Authors
- Haywood, G. W., Anderson, A. J., & Dawes, E. A.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1989-01
- Volume
- 57
- Issue
- 1
- Pages
- 1-6
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W2061742358
- Cited by
- 242
- References
- 1
- FWCI
- 14.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Haywood, G. W., Anderson, A. J., & Dawes, E. A. (1989). The importance of PHB-synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus. FEMS Microbiology Letters. https://doi.org/10.1111/j.1574-6968.1989.tb03210.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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