The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
High efficiency intergeneric conjugal transfer of plasmid DNA from Escherichia coli to methyl DNA-restricting streptomycetes
Abstract
Many streptomycetes, including S. coelicolor A3(2), possess a potent methyl-specific restriction which can present an effective barrier to the introduction of heterologous DNA. We have compared the efficiency of intergeneric conjugal transfer of different types of plasmids to S. coelicolor and S. lividans 66 using two E. coli donors: the standard, methylation proficient strain S17-1, and the methylation deficient donor, ET12567(pUB307). We demonstrate that the methylation deficient donor can yield > 10(4)-fold more S. coelicolor exconjugants than the standard donor. In the case of pSET152 derivatives, which integrate into the host chromosome by site-specific recombination, up to 10% of streptomycete spores in the conjugation mixture inherit the plasmid. The conjugation procedure is efficient enough to obtain exconjugants with 'suicide' delivery plasmids and therefore provides a simple route for conducting gene disruptions in methyl DNA-restricting streptomycetes, and possibly other bacteria.
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
Simon, R., Priefer, U., & Pühler, A. (1983) · Bio/Technology · doi:10.1038/nbt1183-784 · Cited by 7,219
Genetic manipulation of Streptomyces : a laboratory manual
Hopwood, D. A. (1985) · Medical Entomology and Zoology · Cited by 1,782
Chung, C. T., Niemela, S. L., & Miller, R. H. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.7.2172 · Cited by 1,592
Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.
Bierman, M., Logan, R., O'Brien, K., Seno, E. T., Nagaraja Rao, R., & Schoner, B. E. (1992) · Gene · doi:10.1016/0378-1119(92)90627-2 · Cited by 1,402
Genetic Manipulation of Streptomyces — A Laboratory Manual
Parish, J. H. (1986) · Biochemical Education · doi:10.1016/0307-4412(86)90228-1 · Cited by 1,264
Bullock, W. O. (1987) · Medical Entomology and Zoology · Cited by 1,149
MacNeil, D. J., Gewain, K. M., Ruby, C. L., Dezeny, G., Gibbons, P. H., & MacNeil, T. (1992) · Gene · doi:10.1016/0378-1119(92)90603-m · Cited by 729
Elhai, J., Vepritskiy, A., Muro-Pastor, A. M., Flores, E., & Wolk, C. P. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.6.1998-2005.1997 · Cited by 334
Cited by
λ Red-Mediated Genetic Manipulation of Antibiotic-Producing Streptomyces
Gust, B., Chandra, G., Jakimowicz, D., Yuqing, T., Bruton, C. J., & Chater, K. F. (2004) · Advances in Applied Microbiology · doi:10.1016/s0065-2164(04)54004-2 · Cited by 272
Shkoporov, A. N., Efimov, B. A., Khokhlova, E. V., Steele, J. L., Kafarskaia, L. I., & Smeianov, V. V. (2008) · Plasmid · doi:10.1016/j.plasmid.2008.06.005 · Cited by 30
Metadata
- Authors
- Flett, F., Mersinias, V., & Smith, C. P.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-01-17
- Volume
- 155
- Issue
- 2
- Pages
- 223-229
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W2059397761
- Cited by
- 455
- References
- 21
- FWCI
- 13.61
- Type
- journal article
- Language
- en
- Open access
- closed
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- Flett, F., Mersinias, V., & Smith, C. P. (2006). High efficiency intergeneric conjugal transfer of plasmid DNA from Escherichia coli to methyl DNA-restricting streptomycetes. FEMS Microbiology Letters. https://doi.org/10.1111/j.1574-6968.1997.tb13882.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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