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Cited by 84melanin and skin pigmentationCell Adhesion Molecules ResearchMelanoma and MAPK Pathways

Gpnmb is a melanosome-associated glycoprotein that contributes to melanocyte/keratinocyte adhesion in a RGD-dependent fashion

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Tomihari, Mizuki, Hwang, Sun‐Hee, Chung, Jin‐Sung, Cruz Jr., Ponciano D., Ariizumi, Kiyoshi·2009·Experimental Dermatology
DOI 10.1111/j.1600-0625.2008.00830.xPMC PMC2774115
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Abstract

Abstract: Gpnmb is a glycosylated transmembrane protein implicated in the development of glaucoma in mice and melanoma in humans. It shares significant amino acid sequence homology with the melanosome protein Pmel‐17. Its extracellular domain contains a RGD motif for binding to integrin and its intracellular domain has a putative endosomal and/or melanosomal‐sorting motif. These features led us to posit that Gpnmb is associated with melanosomes and involved in cell adhesion. We showed that human Gpnmb is expressed constitutively by melanoma cell lines, primary‐cultured melanocytes and epidermal melanocytes in situ, with most of it found intracellularly within melanosomes and to a lesser degree in lysosomes. Our newly developed monoclonal antibody revealed surface expression of Gpnmb on these pigment cells, albeit to a lesser degree than the intracellular fraction. Gpnmb expression was upregulated by UVA (but not UVB) irradiation and by α‐melanocyte‐stimulating hormone (MSH) (but not β‐MSH); its cell surface expression on melanocytes (but not on melanoma cells) was increased markedly by IFN‐γ and TNF‐α. PAM212 keratinocytes adhered to immobilized Gpnmb in a RGD‐dependent manner. These results indicate that Gpnmb is a melanosome‐associated glycoprotein that contributes to the adhesion of melanocytes with keratinocytes.

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Authors
Tomihari, M., Hwang, S., Chung, J., Cruz Jr., P. D., & Ariizumi, K.
Affiliations
Dallas VA Medical Center (US)
Corresponding author
Ariizumi, Kiyoshi
ORCID
Venue
Experimental Dermatology
Publisher
Wiley
Published
2009-06-04
Volume
18
Issue
7
Pages
586-595
ISSN
  • 0906-6705 (ISSN-L)
  • 1600-0625
DOI
10.1111/j.1600-0625.2008.00830.x
PMC
PMC2774115
OpenAlex
W2017712784
Cited by
84
References
2
Citations by year
2026: 4 · 2025: 8 · 2024: 2 · 2023: 6 · 2022: 2 · 2021: 9 · 2020: 4 · 2019: 3 · 2018: 4 · 2017: 5 · 2016: 3 · 2015: 9 · 2014: 5 · 2013: 6 · 2012: 2
FWCI
2.08
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Experimental Dermatology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health
  • U.S. Public Health Service (A164927-01)
  • National Institute of Allergy and Infectious Diseases (R01AI064927)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
Keywords
GPNMB, melanosomes, RGD motif, integrin binding, melanocytes, keratinocytes, melanoma, UVA irradiation, alpha-melanocyte-stimulating hormone, interferon-gamma, TNF-α, cell surface expression, lysosomes, monoclonal antibodies
MeSH terms
Animals, Cell Adhesion, Cell Line, Cells, Cultured, Eye Proteins, Humans, Melanocytes, Melanoma, Membrane Glycoproteins, Oligopeptides, Skin Neoplasms, Ultraviolet Rays, Keratinocytes, Integrins, Melanosomes, Amino Acid Motifs, Cell Line, Tumor, Mice

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