The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
MicroRNA‐143 reduces viability and increases sensitivity to 5‐fluorouracil in HCT116 human colorectal cancer cells
Abstract
MicroRNAs are aberrantly expressed in cancer; microRNA‐143 (miR‐143) is down‐regulated in colon cancer. HCT116 human colorectal cancer cells were used to investigate the biological role of miR‐143. Transient miR‐143 overexpression resulted in an approximate 60% reduction in cell viability. In addition, stable miR‐143 overexpressing cells were selected with G418 and exposed to 5‐fluorouracil. Increased stable expression of miR‐143 was associated with decreased viability and increased cell death after exposure to 5‐fluorouracil. These changes were associated with increased nuclear fragmentation and caspase ‐3, ‐8 and ‐9 activities. In addition, extracellular‐regulated protein kinase 5, nuclear factor‐κB and Bcl‐2 protein expression was down‐regulated by miR‐143, and further reduced by exposure to 5‐fluorouracil. In conclusion, miR‐143 modulates the expression of key proteins involved in the regulation of cell proliferation, death and chemotherapy response. In addition, miR‐143 increases the sensitivity of colon cancer cells to 5‐fluorouracil, probably acting through extracellular‐regulated protein kinase 5/nuclear factor‐κB regulated pathways. Collectively, the data obtained in the present study suggest anti‐proliferative, chemosensitizer and putative pro‐apoptotic roles for miR‐143 in colon cancer.
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Cites
5-Fluorouracil: mechanisms of action and clinical strategies
Longley, D. B., Harkin, D. P., & Johnston, P. G. (2003) · Nature Reviews Cancer · doi:10.1038/nrc1074 · Cited by 5,332
miR-145 and miR-143 regulate smooth muscle cell fate and plasticity
Cordes, K. R., Sheehy, N. T., White, M. P., Berry, E. C., Morton, S. U., Muth, A. N., Lee, T.-H., Miano, J. M., Ivey, K. N., & Srivastava, D. (2009) · Nature · doi:10.1038/nature08195 · Cited by 1,585
Cited by
5-fluorouracil and other fluoropyrimidines in colorectal cancer: Past, present and future
Vodenkova, S., Buchler, T., Cervena, K., Veskrnova, V., Vodicka, P., & Vymetalkova, V. (2020) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2019.107447 · Cited by 956
Ma, X., Becker Buscaglia, L. E., Barker, J. R., & Li, Y. (2011) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mjr007 · Cited by 542
Blondy, S., David, V., Verdier, M., Mathonnet, M., Perraud, A., & Christou, N. (2020) · Cancer Science · doi:10.1111/cas.14532 · Cited by 507
Delivering the promise of miRNA cancer therapeutics
Pereira, D. M., Rodrigues, P. M., Borralho, P. M., & Rodrigues, C. M. P. (2013) · Drug Discovery Today · doi:10.1016/j.drudis.2012.10.002 · Cited by 288
Self-citationMicroRNA regulation of core apoptosis pathways in cancer
Lima, R. T., Busacca, S., Almeida, G. M., Gaudino, G., Fennell, D. A., & Vasconcelos, M. H. (2011) · European Journal of Cancer · doi:10.1016/j.ejca.2010.11.005 · Cited by 260
Metadata
- Authors
- Borralho, P. M., Kren, B. T., Castro, R. E., Moreira da Silva, I. B., Steer, C. J., & Rodrigues, C. M. P.
- Venue
- The FEBS Journal
- Publisher
- Wiley
- Published
- 2009-10-23
- Volume
- 276
- Issue
- 22
- Pages
- 6689-6700
- ISSN
- 1742-464X (ISSN-L)
- 1742-4658
- OpenAlex
- W1590311193
- Cited by
- 195
- References
- 2
- FWCI
- 4.95
- Type
- journal article
- Language
- en
- Open access
- closed
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- Borralho, P. M., Kren, B. T., Castro, R. E., Moreira da Silva, I. B., Steer, C. J., & Rodrigues, C. M. P. (2009). MicroRNA‐143 reduces viability and increases sensitivity to 5‐fluorouracil in HCT116 human colorectal cancer cells. The FEBS Journal. https://doi.org/10.1111/j.1742-4658.2009.07383.x
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