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Improved Technique for the Fluorimetric Estimation of Catecholamines
Abstract
E uler U. S. v. and F. L ishajko. Improved technique for the fluorimetric estimation of catecholamines. By the addition of small amounts of ethylene diamine (EDA) to the alkali‐ascorbic acid mixture used in the trihydroxyindole (THI) method the discoloration of reaction mixture and instability of fluorescence can be prevented, allowing blanks to maintain their fluorescence values for several hours. The lutines obtained from adrenaline and noradrenaline standards and from alumina eluates are stable over a period of at least one hour. Optimal fluorescence values are obtained when the volume of alkali‐ascorbic‐acid ‐ EDA exceeds the volume of the sample by 1 ml. A proportional increase in fluorescence is observed with sample volumes up to 3 ml. The technique used for estimation of catecholamines in urine and organ extracts is described. The importance of the acidity for the release of catecholamines from conjugates in urine at room temperature is emphasized.
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Cited by
Evidence that angiotensin enhances transmitter release during sympathetic nerve stimulation
HUGHES, J., & ROTH, R. H. (1971) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1971.tb08025.x · Cited by 197
Sex differences in sympathetic-adrenal medullary reactions induced by different stressors
Frankenhaeuser, M., Dunne, E., & Lundberg, U. (1976) · Psychopharmacology · doi:10.1007/bf00428693 · Cited by 173
Wright, M. R., Wright, J. V., & Frankenhaeuser, M. (1981) · Psychophysiology · doi:10.1111/j.1469-8986.1981.tb02467.x · Cited by 78
Sex Differences in the Catecholamine Output of Children
Johansson, G. (1971) · Acta Physiologica Scandinavica · doi:10.1111/j.1748-1716.1971.tb05295.x · Cited by 35
Metadata
- Authors
- Euler, U. S. V., & Lishajko, F.
- Venue
- Acta Physiologica Scandinavica
- Publisher
- Wiley
- Published
- 1961-04
- Volume
- 51
- Issue
- 4
- Pages
- 348-356
- ISSN
- 0001-6772 (ISSN-L)
- 1365-201X
- OpenAlex
- W2061893905
- Cited by
- 978
- References
- 0
- FWCI
- 106.40
- Type
- journal article
- Language
- en
- Open access
- closed
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