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Cited by 48Photodynamic Therapy Research StudiesGlioma Diagnosis and TreatmentPorphyrin Metabolism and Disorders

Deferoxamine Iron Chelation Increases δ‐Aminolevulinic Acid Induced Protoporphyrin IX in Xenograft Glioma Model

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Valdés, Pablo A., Samkoe, Kimberley, O’Hara, Julia A., Roberts, David W., Paulsen, Keith D., Pogue, Brian W.·2010·Photochemistry and Photobiology
DOI 10.1111/j.1751-1097.2009.00664.xPMC PMC2875336
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Abstract

Exogenous administration of δ‐aminolevulinic acid (δ‐ALA) leads to selective accumulation of protoporphyrin IX (PpIX) in brain tumors, and has shown promising results in increasing extent of resection in fluorescence‐guided resection (FGR) of brain tumors. However, this approach still suffers from heterogeneous staining and so some tumor margins may go undetected because of this variation in PpIX production. The aim of this study was to test the hypothesis that iron chelation therapy could increase the level of fluorescence in malignant glioma tumors. Mice implanted with xenograft U251‐GFP glioma tumor cells were given a 200 mg kg−1 dose of deferoxamine (DFO), once a day for 3 days prior to δ‐ALA administration. The PpIX fluorescence observed in the tumor regions was 1.9 times the background in animal group without DFO, and 2.9 times the background on average, in the DFO pre‐treated group. A 50% increase in PpIX fluorescence contrast in the DFO group was observed relative to the control group (t‐test P‐value = 0.0020). These results indicate that iron chelation therapy could significantly increase δ‐ALA‐induced PpIX fluorescence in malignant gliomas, pointing to a potential role of iron chelation therapy for more effective FGR of brain tumors.

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  • DFO significantly enhances ALA-PpIX fluorescence in glioblastoma and skin cancer animal models, although its effect on enhancing ALA-PDT in the skin tumor model is not statistically significant.

    “Furthermore, it significantly enhances ALA-PpIX fluorescence in glioblastoma [89] and skin cancer [69] animal models, although its effect on enhancing ALA-PDT in the skin tumor model is not statistically significant.”

    From 5-Aminolevulinic Acid as a Theranostic Agent for Tumor Fluorescence Imaging and Photodynamic Therapy (2023) · cites this paper as [89]

  • DFO has been demonstrated to enhance PpIX fluorescence and/or PDT effects in various studies with different cell lines including skin, bladder, and glioblastoma tumor cells.

    “DFO has been demonstrated to enhance PpIX fluorescence and/or PDT effects in various studies with different cell lines including skin, bladder, and glioblastoma tumor cells [86,87,88,89,90].”

    From 5-Aminolevulinic Acid as a Theranostic Agent for Tumor Fluorescence Imaging and Photodynamic Therapy (2023) · cites this paper as [89]

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Cited by

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Authors
Valdés, P. A., Samkoe, K., O’Hara, J. A., Roberts, D. W., Paulsen, K. D., & Pogue, B. W.
Affiliations
Dartmouth College (US)
Corresponding author
Pogue, Brian W.
ORCID
Venue
Photochemistry and Photobiology
Publisher
Wiley
Published
2010-03
Volume
86
Issue
2
Pages
471-475
ISSN
  • 0031-8655 (ISSN-L)
  • 1751-1097
DOI
10.1111/j.1751-1097.2009.00664.x
PMC
PMC2875336
OpenAlex
W2055534257
Cited by
48
References
3
Citations by year
2025: 1 · 2024: 4 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 1 · 2019: 5 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 2 · 2014: 4 · 2013: 4 · 2012: 3
FWCI
2.53
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Photochemistry and Photobiology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Cancer Institute (R01CA120368)
  • National Institute of Neurological Disorders and Stroke (R01NS052274)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
  • Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Porphyrin Metabolism and Disorders
Keywords
deferoxamine, iron chelation therapy, 5-aminolevulinic acid, protoporphyrin IX, fluorescence-guided resection, high-grade glioma, brain tumor imaging, tumor margin detection
MeSH terms
Aminolevulinic Acid, Animals, Brain Neoplasms, Deferoxamine, Diagnostic Imaging, Fluorescence, Glioma, Humans, Iron Chelating Agents, Protoporphyrins, Transplantation, Heterologous, Mice

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