The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Is it possible to use expired tubes for routine biochemical analysis in dogs?
Open accessAbstract
Background: Expired collection tubes may be used inadvertently and resampling is not always possible. To date, studies have not been conducted in veterinary medicine to determine whether or not biochemical measurements obtained from specimens collected into expired tubes are accurate enough for clinical decision‐making. Objectives: The aims of this preliminary study were to assess the impact of measuring routine plasma biochemical analytes in canine specimens collected in expired tubes and to investigate the relationship between post‐expiration time and the magnitude of errors. Methods: Blood specimens were collected from 61 dogs and aliquoted equally into tubes containing lithium heparin and gel. One tube was within the expiration date, and the other tube was up to 11 months post‐expiration. Plasma was separated within 1 hour of specimen collection, and concentrations of urea, creatinine, total protein, albumin, total bilirubin, cholesterol, triglycerides, magnesium, calcium, phosphates, sodium, potassium, chloride, total CO 2 , and fructosamine and activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase (ALP), γ‐glutamyltransferase, lactate dehydrogenase, creatine kinase, amylase, and lipase were analyzed immediately and results compared. Results: For most analytes there was no significant difference between results from specimens collected in non‐expired and expired tubes. For ALP and lipase activities and fructosamine and total CO 2 concentrations, significant differences were found, and results obtained for fructosamine and total CO 2 from specimens in expired tubes may have led to erroneous interpretations. The effect of time since expiration was constant over time. Conclusions: When specimens are processed within 1 hour of collection, results of routine biochemical measurements of blood collected in lithium heparin tubes remain clinically valid for up to 11 months after expiration of tubes for the majority of analytes, except for ALP, lipase, fructosamine, and total CO 2 .
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“Pre-evacuated blood collection tubes which are beyond the expiry date have a decreased vacuum which may lead to drawing a less than optimal volume of blood and lead to an improper blood to additive ratio [36], [37].”
From Joint EFLM-COLABIOCLI Recommendation for venous blood sampling (2018) · cites this paper as [37]
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Citations
Cites
Bonini, P., Plebani, M., Ceriotti, F., & Rubboli, F. (2002) · Clinical Chemistry · doi:10.1093/clinchem/48.5.691 · Cited by 850
Errors in clinical laboratories or errors in laboratory medicine?
Plebani, M. (2006) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.2006.123 · Cited by 737
Effects of storage temperature and time on clinical biochemical parameters from rat serum.
Cray, C., Rodriguez, M., Zaias, J., et al. (2009) · PubMed · Cited by 80
The effects of different anticoagulants on routine canine plasma biochemistry
Cerón, J. M. C., Martı́nez-Subiela, S., Hennemann, C. R. D. A., et al. (2003) · The Veterinary Journal · doi:10.1016/j.tvjl.2003.09.009 · Cited by 67
Thoresen, S. I., Havre, G. N., Morberg, H., et al. (1992) · Veterinary Clinical Pathology · doi:10.1111/j.1939-165x.1992.tb00591.x · Cited by 64
Critical differences of clinical chemical parameters in blood from dogs
Jensen, A. L., & Aaes, H. (1993) · Research in Veterinary Science · doi:10.1016/0034-5288(93)90003-x · Cited by 53
Effect of repeated freeze‐thaw cycles on routine plasma biochemical constituents in canine plasma
Reynolds, B., Taillade, B., Médaille, C., et al. (2006) · Veterinary Clinical Pathology · doi:10.1111/j.1939-165x.2006.tb00144.x · Cited by 43
Self-citationStability of common biochemistry analytes in equine blood stored at room temperature
Rendle, D. I., Heller, J., Hughes, K., Innocent, G. T., & Durham, A. E. (2009) · Equine Veterinary Journal · doi:10.2746/042516409x370928 · Cited by 30
Zittel, D., & Hardin, G. G. (2006) · Journal of Analytical Toxicology · doi:10.1093/jat/30.5.317 · Cited by 10
Research works printed but not linked: 2
- Saunier V, 2009, Etude de conservation des échantillons sanguins avant analyse [Study of the effects of blood specimen storage before analysis], Feuillets de Biologie., 291, 41
- Desch G, 1998, Echantillons Biologiques: Phase Préanalytique et Prélèvements en Biologie Médicale [Biological Samples: Pre‐Analytical Phase and Samples in Medical Biology], 22
Cited by
Sorted by citations
Joint EFLM-COLABIOCLI Recommendation for venous blood sampling
Simundic, A.-M., Bölenius, K., Cadamuro, J., Church, S., Cornes, M. P., van Dongen-Lases, E. C., Eker, P., Erdeljanovic, T., Grankvist, K., Guimaraes, J. T., et al. (2018) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2018-0602 · Cited by 240
Metadata
- Authors
- Domingos, M. C., Médaille, C., Concordet, D., & Briend‐Marchal, A.
- Affiliations
- Laboratoire Vebiotel Arcueil France
- École Nationale Vétérinaire de Toulouse (FR)
- Corresponding author
- Domingos, Margarida Canelas
- Venue
- Veterinary Clinical Pathology
- Publisher
- Wiley
- Published
- 2012-05-02
- Volume
- 41
- Issue
- 2
- Pages
- 266-271
- ISSN
- 0275-6382 (ISSN-L)
- 1939-165X
- OpenAlex
- W2098591401
- Cited by
- 9
- References
- 9
- Citations by year
- 2024: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2014: 3 · 2012: 1
- FWCI
- 0.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Veterinary Clinical Pathology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Topics
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Veterinary › Small Animals › Veterinary Medicine and Surgery
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- lithium heparin tubes, dogs, fructosamine, total CO2, alkaline phosphatase, lipase, assay accuracy, sample storage time
- MeSH terms
- Animals, Anticoagulants, Blood Chemical Analysis, Blood Specimen Collection, Dogs, Drug Stability, Drug Storage, Heparin, Reference Values, Specimen Handling, Time Factors
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