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A converged ubiquitin‐proteasome pathway for the degradation of TOC and TOM tail‐anchored receptors
Open accessPublished version of a preprint
Abstract
In plants, thousands of nucleus‐encoded proteins translated in the cytosol are sorted to chloroplasts and mitochondria by binding to specific receptors of the TOC (translocon on the outer chloroplast membrane) and the TOM (translocon on the outer mitochondrial membrane) complexes for import into those organelles. The degradation pathways for these receptors are unclear. Here, we discovered a converged ubiquitin‐proteasome pathway for the degradation of Arabidopsis thaliana TOC and TOM tail‐anchored receptors. The receptors are ubiquitinated by E3 ligase(s) and pulled from the outer membranes by the AAA + adenosine triphosphatase CDC48, after which a previously uncharacterized cytosolic protein, transmembrane domain (TMD)‐binding protein for tail‐anchored outer membrane proteins (TTOP), binds to the exposed TMDs at the C termini of the receptors and CDC48, and delivers these complexes to the 26S proteasome.
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Research works printed but not linked: 1
- 10.1038/s41592-022-01488-1 · DOI · Paper page
Metadata
- Authors
- Yang, M., Chen, S., Lim, S., Yang, L., Zhong, J. Y., Chan, K. C., Zhao, Z., Wong, K., Wang, J., & Lim, B. L.
- Affiliations
- Southern University of Science and Technology (CN)
- Chinese University of Hong Kong (HK)
- University of Hong Kong (HK)
- Corresponding author
- Lim, Boon Leong
- ORCID
- Venue
- Journal of Integrative Plant Biology
- Publisher
- Wiley
- Published
- 2024-03-19
- Volume
- 66
- Issue
- 5
- Pages
- 1007-1023
- ISSN
- 0577-7496 (ISSN-L)
- 1672-9072
- 1744-7909
- OpenAlex
- W4392956512
- Cited by
- 8
- References
- 47
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 1
- FWCI
- 1.09
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Integrative Plant Biology (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion, cc-by-nc-nd)
- Funders
- Science, Technology and Innovation Commission of Shenzhen Municipality (201708183000803)
- Innovation and Technology Fund (Funding Support to State Key Laboratory of Agrobio)
- Research Grants Council, University Grants Committee (Area of Excellence Scheme (AoE/M‐403/16))
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Keywords
- tail-anchored proteins, Toc complex, TOM complex, chloroplast protein import, mitochondrial protein import, ubiquitin-proteasome system, protein degradation, E3 ubiquitin ligase, transmembrane domain, 26S proteasome, Arabidopsis thaliana, nuclear-encoded chloroplast proteins
- MeSH terms
- Valosin Containing Protein, Arabidopsis, Ubiquitin, Arabidopsis Proteins, Proteasome Endopeptidase Complex, Proteolysis
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