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Cited by 28Marine Toxins and Detection MethodsMarine Plankton and BiogeochemistryAquatic Ecosystems and Phytoplankton Dynamics

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Characterization of Dinophysis spp. (Dinophyceae, Dinophysiales) from the mid‐Atlantic region of the United States1

Wolny, Jennifer L., Egerton, Todd A., Handy, Sara M., Stutts, Whitney L., Smith, Juliette L., Whereat, Edward B., Bachvaroff, Tsvetan R., Henrichs, Darren W., Campbell, Lisa, Deeds, Jonathan R.·2020·Journal of Phycology
DOI 10.1111/jpy.12966
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Abstract

Due to the increasing prevalence of Dinophysis spp. and their toxins on every US coast in recent years, the need to identify and monitor for problematic Dinophysis populations has become apparent. Here, we present morphological analyses, using light and scanning electron microscopy, and rDNA sequence analysis, using a ~2‐kb sequence of ribosomal ITS 1, 5.8S, ITS 2, and LSU DNA, of Dinophysis collected in mid‐Atlantic estuarine and coastal waters from Virginia to New Jersey to better characterize local populations. In addition, we analyzed for diarrhetic shellfish poisoning (DSP) toxins in water and shellfish samples collected during blooms using liquid‐chromatography tandem mass spectrometry and an in vitro protein phosphatase inhibition assay and compared this data to a toxin profile generated from a mid‐Atlantic Dinophysis culture. Three distinct morphospecies were documented in mid‐Atlantic surface waters: D. acuminata, D. norvegica, and a “small Dinophysis sp.” that was morphologically distinct based on multivariate analysis of morphometric data but was genetically consistent with D. acuminata. While mid‐Atlantic D. acuminata could not be distinguished from the other species in the D. acuminata‐complex (D. ovum from the Gulf of Mexico and D. sacculus from the western Mediterranean Sea) using the molecular markers chosen, it could be distinguished based on morphometrics. Okadaic acid, dinophysistoxin 1, and pectenotoxin 2 were found in filtered water and shellfish samples during Dinophysis blooms in the mid‐Atlantic region, as well as in a locally isolated D. acuminata culture. However, DSP toxins exceeded regulatory guidance concentrations only a few times during the study period and only in noncommercial shellfish samples.

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  • Common marine and estuarine HAB species in Bay waters (A. monilatum, D. acuminata, K. veneficum, M. polykrikoides, P. minimum) have historically been present but are increasing in abundance and extent.

    “Marshall and Egerton (2009a,b), Li et al. (2015), and Wolny et al. (2020) have summarized common marine and estuarine HAB species in Bay waters that have historically been present but are increasing in abundance and extent: Alexandrium monilatum, Dinophysis acuminata, Karlodinium veneficum, Margalefidinium polykrikoides, and Prorocentrum minimum.”

    From Current and Future Remote Sensing of Harmful Algal Blooms in the Chesapeake Bay to Support the Shellfish Industry (2020) · cites this paper as Wolny et al. (2020)

  • To date, the toxicity of Dinophysis spp. in Maryland and Virginia waters appears to be minimal compared to other regions.

    “To date, the toxicity of Dinophysis spp. in Maryland and Virginia waters appears to be minimal compared to other regions (Wolny et al., 2020).”

    From Current and Future Remote Sensing of Harmful Algal Blooms in the Chesapeake Bay to Support the Shellfish Industry (2020) · cites this paper as Wolny et al., 2020

  • Detection of Dinophysis acuminata at the low concentrations typical in the Chesapeake Bay region (average 403 cells/L) may be aided by using its prey items as a proxy.

    “The detection of D. acuminata within the phytoplankton community at the low concentrations it is typically found in the Chesapeake Bay region (average of 403 cells•L -1 ; Wolny et al., 2020), may be aided by using its prey items as a proxy.”

    From Current and Future Remote Sensing of Harmful Algal Blooms in the Chesapeake Bay to Support the Shellfish Industry (2020) · cites this paper as Wolny et al., 2020

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Metadata

Authors
Wolny, J. L., Egerton, T. A., Handy, S. M., Stutts, W. L., Smith, J. L., Whereat, E. B., Bachvaroff, T. R., Henrichs, D. W., Campbell, L., & Deeds, J. R.
Affiliations
  • Maryland Department of Natural Resources (US)
  • Old Dominion University (US)
  • Center for Food Safety and Applied Nutrition (US)
  • William & Mary (US)
  • University of Delaware (US)
  • University of Maryland, Baltimore (US)
  • Texas A&M University (US)
Corresponding author
Wolny, Jennifer L.
ORCID
Venue
Journal of Phycology
Publisher
Wiley
Published
2020-02-10
Volume
56
Issue
2
Pages
404-424
ISSN
  • 0022-3646 (ISSN-L)
  • 1529-8817
DOI
10.1111/jpy.12966
OpenAlex
W3000500183
Cited by
28
References
0
Citations by year
2025: 5 · 2024: 4 · 2023: 5 · 2022: 7 · 2021: 3 · 2020: 4
FWCI
1.76
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Journal of Phycology (publishedVersion)
  • PubMed (publishedVersion)
  • NOAA Institutional Repository (submittedVersion)
Funders
  • Texas Sea Grant, Texas A and M University (NA14OAR4170102)
  • National Oceanic and Atmospheric Administration (NA14OAR4170093)
  • National Science Foundation (EF‐0629624)
  • Virginia Department of Health
  • Virginia Department of Environmental Quality
Topics
  • Physical Sciences › Environmental Science › Environmental Chemistry › Marine Toxins and Detection Methods
  • Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine Plankton and Biogeochemistry
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
Keywords
Dinophysis, Dinophysis acuminata, okadaic acid, dinophysistoxin-1, pectenotoxin-2, harmful algal blooms, morphospecies, morphometrics, ITS sequences, shellfish contamination, toxin monitoring
MeSH terms
Dinoflagellida, Marine Toxins, Mediterranean Sea, Mid-Atlantic Region, Gulf of Mexico, Gulf of America

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