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Cited by 45Lipid metabolism and biosynthesisAlgal biology and biofuel productionCancer, Lipids, and Metabolism

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Long‐chain acyl‐CoA synthetases activate fatty acids for lipid synthesis, remodeling and energy production in Chlamydomonas

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Bai, Fan, Yu, Lihua, Shi, Jianan, Li‐Beisson, Yonghua, Liu, Jin·2022·New Phytologist
DOI 10.1111/nph.17813
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Abstract

Summary: Long‐chain acyl‐CoA synthetases (LACSs) play many roles in mammals, yeasts and plants, but knowledge on their functions in microalgae remains fragmented. Here via genetic, biochemical and physiological analyses, we unraveled the function and roles of LACSs in the model microalga Chlamydomonas reinhardtii. In vitro assays on purified recombinant proteins revealed that CrLACS1, CrLACS2 and CrLACS3 all exhibited bona fide LACS activities toward a broad range of free fatty acids. The Chlamydomonas mutants compromised in CrLACS1, CrLACS2 or CrLACS3 did not show any obvious phenotypes in lipid content or growth under nitrogen (N)‐replete condition. But under N‐deprivation, CrLACS1 or CrLACS 2 suppression resulted in c. 50% less oil, yet with a higher amount of chloroplast lipids. By contrast, CrLACS3 suppression impaired oil remobilization and cell growth severely during N‐recovery, supporting its role in fatty acid β‐oxidation to provide energy and carbon sources for regrowth. Transcriptomics analysis suggested that the observed lipid phenotypes are likely not due to transcriptional reprogramming but rather a shift in metabolic adjustment. Taken together, this study provided solid experimental evidence for essential roles of the three Chlamydomonas LACS enzymes in lipid synthesis, remodeling and catabolism, and highlighted the importance of lipid homeostasis in cell growth under nutrient fluctuations.

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  • Analysis of gene expression involved in lipid production at the transcriptome level in microalgae has offered valuable insights into metabolic pathways and contextual information regarding genomic-level modifications in algae.

    “In recent years, analysis of gene expression involved in lipid production at the transcriptome level in microalgae has offered valuable insights into metabolic pathways, as well as contextual information regarding modifications at the genomic level in algae [231–234].”

    From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [233]

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Research works printed but not linked: 67

  1. 10.1016/j.plaphy.2021.01.042 · DOI
  2. 10.1105/tpc.113.117580 · DOI
  3. 10.1016/S0021-9258(19)74070-8 · DOI
  4. 10.1074/jbc.M111.334052 · DOI
  5. 10.1093/jn/132.8.2123 · DOI
  6. 10.3390/molecules23102583 · DOI
  7. 10.1023/A:1006823831984 · DOI
  8. 10.1074/jbc.M100884200 · DOI
  9. 10.1105/tpc.019646 · DOI
  10. 10.1046/j.1365-313X.2002.01405.x · DOI
  11. 10.1016/j.plefa.2014.02.007 · DOI
  12. 10.1128/EC.00013-14 · DOI
  13. 10.1016/j.plaphy.2013.10.036 · DOI
  14. 10.1002/j.1460-2075.1996.tb00755.x · DOI · Paper page
  15. 10.1111/j.1365-313X.2008.03492.x · DOI · Paper page
  16. 10.1016/S0022-2275(20)41656-6 · DOI
  17. 10.1016/j.semcdb.2020.02.014 · DOI
  18. 10.1111/j.1365-313X.2011.04722.x · DOI
  19. 10.1104/pp.114.250365 · DOI
  20. 10.1186/s13068-016-0598-7 · DOI
  21. 10.1083/jcb.127.3.751 · DOI
  22. 10.1042/BJ20070449 · DOI
  23. 10.1073/pnas.87.3.1228 · DOI
  24. 10.1074/jbc.270.18.10861 · DOI
  25. 10.1105/tpc.18.00361 · DOI
  26. 10.1111/tpj.13498 · DOI
  27. 10.1093/pcp/pcz022 · DOI
  28. 10.1007/s00253-014-6354-7 · DOI
  29. 10.1073/pnas.2005600117 · DOI
  30. 10.1016/j.biotechadv.2020.107545 · DOI
  31. 10.1371/journal.pbio.1002053 · DOI
  32. 10.1186/s13068-018-1332-4 · DOI
  33. 10.1128/EC.00268-12 · DOI
  34. 10.1105/tpc.15.00465 · DOI
  35. 10.1111/tpj.12787 · DOI · Paper page
  36. 10.1111/tpj.13143 · DOI
  37. 10.1016/j.biortech.2016.04.112 · DOI · Paper page
  38. 10.1111/tpj.14302 · DOI
  39. 10.1186/s13068-015-0391-z · DOI
  40. 10.1111/j.1365-313X.2009.03892.x · DOI
  41. 10.1016/j.biochi.2020.04.022 · DOI
  42. 10.1186/s13068-019-1366-2 · DOI
  43. 10.1016/j.copbio.2011.12.001 · DOI
  44. 10.1104/pp.110.165159 · DOI · Paper page
  45. 10.1128/EC.00203-09 · DOI
  46. 10.1002/pmic.201100114 · DOI
  47. 10.1111/tpj.12747 · DOI
  48. 10.1016/j.plipres.2016.05.002 · DOI
  49. 10.1104/pp.003251 · DOI
  50. 10.1021/acs.jafc.0c04899 · DOI
  51. 10.1104/pp.003269 · DOI
  52. Tang Y, 2016, Microalgae as a feedstock for biofuel precursors and value‐added products: green fuels and golden opportunities, BioResources, 11, 2850
  53. 10.1038/nprot.2012.016 · DOI
  54. 10.1186/s13068-014-0171-1 · DOI
  55. 10.1016/j.gpb.2019.01.003 · DOI · Paper page
  56. 10.1093/pcp/pcz037 · DOI
  57. 10.1016/j.bbadis.2012.02.010 · DOI
  58. 10.1007/s00425-010-1110-4 · DOI
  59. 10.1021/acs.jafc.0c01284 · DOI
  60. 10.1105/tpc.112.100701 · DOI
  61. 10.1007/s10811-011-9707-y · DOI
  62. 10.1105/tpc.114.124099 · DOI
  63. 10.1186/s13068-021-01969-z · DOI
  64. 10.3389/fpls.2021.640996 · DOI
  65. 10.1093/pcp/pcz019 · DOI
  66. 10.1111/j.1365-313X.2010.04396.x · DOI
  67. 10.1074/jbc.M210557200 · DOI

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Metadata

Authors
Bai, F., Yu, L., Shi, J., Li‐Beisson, Y., & Liu, J.
Affiliations
  • Peking University (CN)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
Liu, Jin
ORCID
Venue
New Phytologist
Publisher
Wiley
Published
2021-11-05
Volume
233
Issue
2
Pages
823-837
ISSN
  • 0028-646X (ISSN-L)
  • 1469-8137
DOI
10.1111/nph.17813
OpenAlex
W3205017555
Cited by
45
References
1
Citations by year
2026: 5 · 2025: 12 · 2024: 18 · 2023: 5 · 2022: 5
FWCI
3.41
Type
journal article
Language
en
Open access
Licence
Locations
  • New Phytologist (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
Funders
National Natural Science Foundation of China (31770048)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
Keywords
long-chain acyl-CoA synthetase, Chlamydomonas reinhardtii, fatty acid activation, lipogenesis, lipid remodeling, nitrogen starvation, nitrogen recovery, fatty acid beta-oxidation, lipid homeostasis, chloroplast lipids, microalgae
MeSH terms
Chlamydomonas, Coenzyme A, Fatty Acids, Ligases, Chlamydomonas reinhardtii

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