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Cited by 21Plant nutrient uptake and metabolismPlant Molecular Biology ResearchNitrogen and Sulfur Effects on Brassica

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Identification of vacuolar phosphate influx transporters in Brassica napus

Open access
Han, Bei, Wang, Chuang, Wu, Tao, Yan, Junjun, Jiang, Aosheng, Liu, Yu, Luo, Yu, Cai, Hongmei, Ding, Guangda, Dong, Xu, White, Philip J., Xu, Fangsen, Wang, Sheliang, Shi, Lei·2022·Plant, Cell & Environment
DOI 10.1111/pce.14423

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Abstract

Recent progress has shown that vacuolar Pi transporters (VPTs) are important for cellular Pi homoeostasis in Arabidopsis thaliana and Oryza sativa under fluctuating external Pi supply, but the identity and involvement of VPTs in cellular Pi homoeostasis in Brassica napus is poorly understood. Here, we identified two vacuolar Pi influx transporters B. napus, BnA09PHT5;1b and BnCnPHT5;1b, and uncovered their necessity for cellular Pi homoeostasis through functional analysis. Both Brassica proteins are homologs of Arabidopsis AtPHT5;1 with a similar sequence, structure, tonoplast localization, and VPT activity. Brassica pht5;1b double mutants had smaller shoots and larger shoot cellular Pi concentrations than wild‐type B. napus, which contrasts with a previous study of the Arabidopsis pht5;1 mutant, suggesting that PHT5;1‐VPTs play different roles in cellular Pi homoeostasis in seedlings of B. napus and A. thalian a. Disruption of BnPHT5;1b genes also caused Pi toxicity in floral organs, reduced seed yield and impacted seed traits, consistent with the proposed role of AtPHT5;1 in floral Pi homoeostasis in Arabidopsis. Taken together, our studies identified two vacuolar Pi influx transporters in B. napus and revealed the distinct and conserved roles of BnPHT5;1bs in cellular Pi homoeostasis in this plant species.

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Metadata

Authors
Han, B., Wang, C., Wu, T., Yan, J., Jiang, A., Liu, Y., Luo, Y., Cai, H., Ding, G., Dong, X., White, P. J., Xu, F., Wang, S., & Shi, L.
Affiliations
  • Huazhong Agricultural University (CN)
  • Zhejiang University (CN)
  • Innovation Academy for Precision Measurement Science and Technology, CAS (CN)
  • James Hutton Institute (GB)
Corresponding author
  • Wang, Sheliang
  • Shi, Lei
ORCID
Venue
Plant, Cell & Environment
Publisher
Wiley
Published
2022-09
Volume
45
Issue
11
Pages
3338-3353
ISSN
  • 0140-7791 (ISSN-L)
  • 1365-3040
DOI
10.1111/pce.14423
OpenAlex
W4292543007
Cited by
21
References
1
Citations by year
2026: 3 · 2025: 5 · 2024: 7 · 2023: 5 · 2022: 1
FWCI
2.48
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Plant Cell & Environment (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China (32172662, 31972498)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Nitrogen and Sulfur Effects on Brassica
Keywords
Brassica napus, tonoplast, phosphate toxicity, floral organs, seed yield, seed traits, double mutants, Arabidopsis thaliana homologs
MeSH terms
Brassica, Phosphates, Plant Proteins, Vacuoles, Arabidopsis, Gene Expression Regulation, Plant, Phosphate Transport Proteins, Brassica napus

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CoChat. (2026). Citation check: Identification of vacuolar phosphate influx transporters in Brassica napus. https://app.cochat.ai/papers/10.1111/pce.14423
The paper
Han, B., Wang, C., Wu, T., Yan, J., Jiang, A., Liu, Y., Luo, Y., Cai, H., Ding, G., Dong, X., White, P. J., Xu, F., Wang, S., & Shi, L. (2022). Identification of vacuolar phosphate influx transporters in Brassica napus. Plant, Cell & Environment. https://doi.org/10.1111/pce.14423