Chlamydia pneumoniae and Chlamydia Trachomatis Infection Differentially Modulates Human Dendritic Cell Line (MUTZ) Differentiation and Activation
Open accessAbstract
Chlamydia trachomatis and Chlamydia pneumoniae are important human pathogens that infect the urogenital/anorectal and respiratory tracts, respectively. Whilst the ability of these bacteria to infect epithelia is well defined, there is also considerable evidence of infection of leucocytes, including dendritic cells (DC s). Using a human dendritic cell line (MUTZ), we demonstrate that the infection and replication of chlamydiae inside DC s is species and serovar specific and that live infection with C. pneumoniae is required to upregulate costimulatory markers CD 80, CD 83 and human leucocyte antigen (HLA)‐DR on MUTZ cells, as well as induce secretion of interleukin (IL)‐2, IL‐6, IL‐8, IL‐12 (p70), interferon‐gamma and tumour necrosis factor‐alpha Conversely, C. trachomatis serovar D failed to upregulate DC costimulatory markers, but did induce secretion of high concentrations of IL‐8. Interestingly, we also observed that infection of MUTZ cells with C. pneumoniae or C. trachomatis serovar L2, whilst not replicative, remained infectious and upregulated lymph node migratory marker CCR 7 mRNA. Taken together, these data confirm the findings of other groups using primary DC s and demonstrate the utility of MUTZ cells for further studies of chlamydial infection.
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C. pneumoniae EBs can become internalized into host cells, including many phagocytes.
“C. pneumoniae EBs can become internalized into host cells, including many phagocytes1,37,38.”
From Chlamydia pneumoniae can infect the central nervous system via the olfactory and trigeminal nerves and contributes to Alzheimer’s disease risk (2022) · cites this paper as [37]
The Results section reports internalized C. pneumoniae elementary bodies in iMUTZ dendritic cells, supporting uptake by a host phagocyte. The Introduction describes infection of several immune-cell types by Chlamydia species generally, but does not establish that C. pneumoniae EBs enter many types of phagocytes specifically. Page numbers are unavailable in our copy. (LLM, conf=0.82)
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Chacko, A., Delbaz, A., Walkden, H., Basu, S., Armitage, C. W., Eindorf, T., Trim, L. K., Miller, E., West, N. P., St John, J. A., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-06749-9 · Cited by 65 · see citing works
Self-citation
- Authors
- Armitage, C. W., O'Meara, C. P., & Beagley, K. W.
- Affiliations
- Queensland University of Technology (AU)
- Institute of Health and Biomedical Innovation Queensland University of Technology Kelvin Grove Qld Australia
- Corresponding author
- Beagley, K. W.
- Venue
- Scandinavian Journal of Immunology
- Publisher
- Wiley
- Published
- 2015-06-18
- Volume
- 82
- Issue
- 1
- Pages
- 48-54
- ISSN
- 0300-9475 (ISSN-L)
- 1365-3083
- OpenAlex
- W2078834725
- Cited by
- 8
- References
- 1
- Citations by year
- 2024: 2 · 2023: 2 · 2022: 1 · 2020: 1 · 2018: 1 · 2017: 1
- FWCI
- 0.51
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Scandinavian Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Westmead Millennium Institute for Medical Research
- Vrije Universiteit Amsterdam
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- Chlamydia pneumoniae, Chlamydia trachomatis, dendritic cells, dendritic cell differentiation, dendritic cell activation, interleukin-8, CCR7, lymph node migration, interferon-gamma
- MeSH terms
- CD83 Antigen, Cell Line, Chlamydia Infections, Chlamydia trachomatis, Dendritic Cells, Epithelial Cells, HLA-DR Antigens, Humans, Immunoglobulins, Interferon-gamma, Interleukin-2, Membrane Glycoproteins, Tumor Necrosis Factor-alpha, Antigens, CD, Interleukin-6, Interleukin-8, Chlamydophila pneumoniae, B7-1 Antigen, Interleukin-12, Chlamydophila Infections, Receptors, CCR7
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