The glucosinolate breakdown product indole‐3‐carbinol acts as an auxin antagonist in roots of A rabidopsis thaliana
Open accessAbstract
The glucosinolate breakdown product indole-3-carbinol functions in cruciferous vegetables as a protective agent against foraging insects. While the toxic and deterrent effects of glucosinolate breakdown on herbivores and pathogens have been studied extensively, the secondary responses that are induced in the plant by indole-3-carbinol remain relatively uninvestigated. Here we examined the hypothesis that indole-3-carbinol plays a role in influencing plant growth and development by manipulating auxin signaling. We show that indole-3-carbinol rapidly and reversibly inhibits root elongation in a dose-dependent manner, and that this inhibition is accompanied by a loss of auxin activity in the root meristem. A direct interaction between indole-3-carbinol and the auxin perception machinery was suggested, as application of indole-3-carbinol rescues auxin-induced root phenotypes. In vitro and yeast-based protein interaction studies showed that indole-3-carbinol perturbs the auxin-dependent interaction of Transport Inhibitor Response (TIR1) with auxin/3-indoleacetic acid (Aux/IAAs) proteins, further supporting the possibility that indole-3-carbinol acts as an auxin antagonist. The results indicate that chemicals whose production is induced by herbivory, such as indole-3-carbinol, function not only to repel herbivores, but also as signaling molecules that directly compete with auxin to fine tune plant growth and development.
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In vitro, indole-3-carbinol interferes with interaction between auxin and its receptor TIR1 by binding at an allosteric site.
“In vitro, indole-3-carbinol interferes with the interaction between auxin and its receptor TIR1 by binding at an allosteric site (Katz et al., 2015).”
From Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy (2020) · cites this paper as Katz et al., 2015
The in vitro pull-down results in “I3C acts as an auxin antagonist” show that I3C inhibits the auxin-dependent interaction between TIR1 and IAA3, not a directly measured interaction between auxin and TIR1. The same section’s molecular model predicts that I3C may compete with auxin at TIR1’s auxin-binding site; it does not support binding at an allosteric site. (LLM, conf=0.88)
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Cites
Trott, O., & Olson, A. J. (2010) · Journal of Computational Chemistry · doi:10.1002/jcc.21334 · Cited by 38,412 · see citing works
The F-box protein TIR1 is an auxin receptor
Dharmasiri, N., Dharmasiri, S., & Estelle, M. (2005) · Nature · doi:10.1038/nature03543 · Cited by 2,083 · see citing works
Mechanism of auxin perception by the TIR1 ubiquitin ligase
Tan, X., Calderon-Villalobos, L. I. A., Sharon, M., Zheng, C., Robinson, C. V., Estelle, M., & Zheng, N. (2007) · Nature · doi:10.1038/nature05731 · Cited by 1,688 · see citing works
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Research works printed but not linked: 6
- Bogdanove, A.J. and Martin, G.B. (2000) AvrPto-dependent Pto-interacting proteins and AvrPto-interacting proteins in tomato. Proc. Natl Acad. Sci. USA, 97, 8836-8840.
- Bradlow, H.L. (2008) Review. Indole-3-carbinol as a chemoprotective agent in breast and prostate cancer. In Vivo, 22, 441-445.
- Hull, A.K., Vij, R. and Celenza, J.L. (2000) Arabidopsis cytochrome P450s that catalyze the first step of tryptophan-dependent indole-3-acetic acid biosynthesis. Proc. Natl Acad. Sci. USA, 97, 2379-2384.
- McDanell, R., McLean, A.E., Hanley, A.B., Heaney, R.K. and Fenwick, G.R. (1988) Chemical and biological properties of indole glucosinolates (glu- cobrassicins): a review. Food Chem. Toxicol. 26, 59-70.
- Parry, G., Calderon-Villalobos, L.I., Prigge, M., Peret, B., Dharmasiri, S., Itoh, H., Lechner, E., Gray, W.M., Bennett, M. and Estelle, M. (2009) Complex regulation of the TIR1/AFB family of auxin receptors. Proc. Natl Acad. Sci. USA, 106, 22540-22545.
- Weigel, D. and Glazebrook, J. (2002) Arabidopsis: A Laboratory Manual. Cold Sping Harbor, NY: Cold Spring Harbor Laboratory Press.
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Erb, M., & Kliebenstein, D. J. (2020) · Plant Physiology · doi:10.1104/pp.20.00433 · Cited by 1,333 · see citing works
Salehin, M., Li, B., Tang, M., Katz, E., Song, L., Ecker, J. R., Kliebenstein, D. J., & Estelle, M. (2019) · Nature Communications · doi:10.1038/s41467-019-12002-1 · Cited by 311 · see citing works
Self-citation
- Authors
- Katz, E., Nisani, S., Yadav, B. S., Woldemariam, M. G., Shai, B., Obolski, U., Ehrlich, M., Shani, E., Jander, G., & Chamovitz, D. A.
- Affiliations
- Tel Aviv University (IL)
- Boyce Thompson Institute (US)
- Corresponding author
- Chamovitz, Daniel A.
- ORCID
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2015-04-13
- Volume
- 82
- Issue
- 4
- Pages
- 547-555
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2042406650
- Cited by
- 118
- References
- 3
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 11 · 2023: 14 · 2022: 9 · 2021: 18 · 2020: 20 · 2019: 13 · 2018: 5 · 2017: 7 · 2016: 4 · 2015: 1 · 2012: 1
- FWCI
- 6.86
- Type
- journal article
- Language
- en
- Update notices
- addendum (2015-09-16)
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Binational Agricultural Research and Development Fund (IS-4505-12R)
- PBC Fellowship Program for Outstanding Post-Doctoral Researchers
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- indole-3-carbinol, glucosinolate breakdown products, auxin antagonists, auxin signaling, root elongation inhibition, root meristem, TIR1, Aux/IAA proteins, plant growth regulation, Arabidopsis thaliana
- MeSH terms
- Glucosinolates, Indoleacetic Acids, Indoles, Arabidopsis, Gene Expression Regulation, Plant, Plant Roots, Arabidopsis Proteins
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