CoChat
Sign Up
Cited by 24Ion Transport and Channel RegulationRenin-Angiotensin System StudiesBlood Pressure and Hypertension Studies

The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.

The renal cortical collecting duct: a secreting epithelium?

Open access
Morla, Luciana, Doucet, Alain, Lamouroux, Christine, Crambert, Gilles, Edwards, Aurélie·2016·The Journal of Physiology
DOI 10.1113/jp272877PMC PMC5063930
Open this paper in CoChat — free account

Abstract

Key points The cortical collecting duct (CCD) plays an essential role in sodium homeostasis by fine‐tuning the amount of sodium that is excreted in the urine. Ex vivo, the microperfused CCD reabsorbs sodium in the absence of lumen‐to‐bath concentration gradients. In the present study, we show that, in the presence of physiological lumen‐to‐bath concentration gradients, and in the absence of endocrine, paracrine and neural regulation, the mouse CCD secretes sodium, which represents a paradigm shift. This secretion occurs via the paracellular route, as well as a transcellular pathway that is energized by apical H+/K+‐ATPase type 2 pumps operating as Na+/K+ exchangers. The newly identified transcellular secretory pathway represents a physiological target for the regulation of sodium handling and for anti‐hypertensive therapeutic agents. Abstract In vitro microperfusion experiments have demonstrated that cortical collecting ducts (CCDs) reabsorb sodium via principal and type B intercalated cells under sodium‐depleted conditions and thereby contribute to sodium and blood pressure homeostasis. However, these experiments were performed in the absence of the transepithelial ion concentration gradients that prevail in vivo and determine paracellular transport. The present study aimed to characterize Na+, K+ and Cl− fluxes in the mouse CCD in the presence of physiological transepithelial concentration gradients. For this purpose, we combined in vitro measurements of ion fluxes across microperfused CCDs of sodium‐depleted mice with the predictions of a mathematical model. When NaCl transport was inhibited in all cells, CCDs secreted Na+ and reabsorbed K+; Cl− transport was negligible. Removing inhibitors of type A and B intercalated cells increased Na+ secretion in wild‐type (WT) mice but not in H+/K+‐ATPase type 2 (HKA2) knockout mice. Further inhibition of basolateral NaCl entry via the Na+‐K+‐2Cl− cotransporter in type A intercalated cells reduced Na+ secretion in WT mice to the levels observed in HKA2−/− mice. With no inhibitors, WT mouse CCDs still secreted Na+ and reabsorbed K+. In vivo, HKA2−/− mice excreted less Na+ than WT mice after switching to a high‐salt diet. Taken together, our results indicate that type A intercalated cells secrete Na+ via basolateral Na+‐K+‐2Cl− cotransporters in tandem with apical HKA2 pumps. They also suggest that the CCD can mediate overall Na+ secretion, and that its ability to reabsorb NaCl in vivo depends on the presence of acute regulatory factors.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1113/jp272877

Cites

60 of 60 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Research works printed but not linked: 1

  1. Schlatter E, 1988, Electrophysiological studies in intercalated cells of rat cortical collecting tubules, Pflügers Archiv, 411, R101

Cited by

Sorted by citations

See all 24

Related

See all

Metadata

Authors
Morla, L., Doucet, A., Lamouroux, C., Crambert, G., & Edwards, A.
Affiliations
  • Inserm (FR)
  • Centre de Recherche des Cordeliers (FR)
Corresponding author
Edwards, Aurélie
ORCID
Venue
The Journal of Physiology
Publisher
Wiley
Published
2016-08-13
Volume
594
Issue
20
Pages
5991-6008
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1113/jp272877
PMC
PMC5063930
OpenAlex
W2508550362
Cited by
24
References
60
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 1
FWCI
1.21
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Blood Pressure and Hypertension Studies
Keywords
sodium secretion, paracellular transport, Na+-K+-2Cl- cotransporter, type A intercalated cells, principal cells, sodium homeostasis, blood pressure regulation, high-salt diet, antihypertensive therapy
MeSH terms
Sodium-Potassium-Exchanging ATPase, Animals, Biological Transport, Chlorides, Epithelium, Kidney Tubules, Collecting, Male, Mice, Inbred C57BL, Potassium, Sodium, Mice, Knockout, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review