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Cited by 14Periodontal Regeneration and TreatmentsTendon Structure and TreatmentKnee injuries and reconstruction techniques

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Can Platelet rich plasma stimulate human ACL growth in culture? A preliminary experience

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Singh Dhillon, Mandeep·2015·Muscles, Ligaments and Tendons Journal
DOI 10.11138/mltj/2015.5.3.156PMC PMC4617214
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Abstract

INTRODUCTION: Platelet Rich Plasma (PRP) contains numerous growth factors; Platelet poor plasma (PPP) is plasma proteins without platelets, containing growth factors other than platelet derived. We planned to evaluate the effect of both autologous PRP & PPP on human ACL cell growth characteristics in culture conditions to see if one was better than the other. METHODS: ACL remnants were collected from eleven patients during ACL reconstruction surgery; PPP and PRP were prepared from blood of these patients. Cells were isolated, identified and cultured and were then divided into six groups. Groups A-D had Fetal Bovine Serum (FBS) added to them along with different concentrations of PRP and PPP. Groups E and F had 5% and 10% PRP respectively but lacked FBS. Cell viability was assayed by MTT and Annexin V assay, and DNA content was evaluated by propidium iodide staining and flow cytometry. RESULTS: analysis of cultured cells showed that addition of PRP (5 or 10%) increased the viability of ACL cells in 4 out of 11 and promoted cell proliferation in 8 of 11 donor samples; 10% PRP was more effective than 5% PRP. However, the difference in effectiveness of 10% PRP was not significantly better than 5% PRP. 5% PPP had no significant effect on cell viability, but it led to an increase in DNA content in 5 of 11. There was no statistically significant effect of either PRP or PPP in preventing cell death (depicted by apoptosis rate). CONCLUSION: PRP may have an enhancing effect on ACL cell viability and promotion of cell proliferation but the ideal concentration of PRP for these positive effects needs to be determined before it could be used in clinical settings for enhancing primary repair of torn ACL. Also larger, more controlled and better studies are needed to confirm its clinical utility.

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References 26 · Cited by 14

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  1. [1]Rudolph KS, Axel MJ, Buchanan TS, et al. Dynamic stability in the anterior cruciate ligament deficient knee. Knee Surg Sports Traumatol Arthrosc. 2001;9:62–71. doi: 10.1007/s001670000166. [DOI] [PubMed] [Google Scholar] · DOI
  2. [2]Li G, Papannagari R, Defrate LE, et al. The effects of ACL deficiency on mediolateral translation and varus-valgus rotation. ActaOrthop. 2007;78:355–360. doi: 10.1080/17453670710013924. [DOI] [PubMed] [Google Scholar] · DOI
  3. [3]Daniel DM, Stone ML, Dobson BE, et al. Fate of the ACL injured patient: A prospective outcome study. Am J Sports Med. 1994;22:632–644. doi: 10.1177/036354659402200511. [DOI] [PubMed] [Google Scholar] · DOI
  4. [4]Nin JR, Gasque GM, Azcárate AV, Beola JD, Gonzalez MH. Has platelet-rich plasma any role in anterior cruciate ligament allograft healing? Arthroscopy. 2009;25(11):1206–1213. doi: 10.1016/j.arthro.2009.06.002. [DOI] [PubMed] [Google Scholar] · DOI
  5. [5]Dhillon MS, Bali K, Prabhakar S. Differences among mechanoreceptors in healthy and injured anterior cruciate ligaments and their clinical importance. Muscles Ligaments Tendons J. 2012;2(1):38–43. [PMC free article] [PubMed] [Google Scholar]
  6. [6]O’Donoghue DH, Rockwood CA, Jr, Frank GR, Jack SC, Kenyon R. Repair of the anterior cruciate ligament in dogs. J Bone Joint Surg Am. 1966;48:503–519. [PubMed] [Google Scholar]
  7. [7]Feagin JA, Jr, Curl WW. Isolated tear of the anterior cruciate ligament: 5-Year follow-up study. Am J Sports Med. 1976;4:95–100. doi: 10.1177/036354657600400301. [DOI] [PubMed] [Google Scholar] · DOI
  8. [8]Marshall JL, Warren RF, Wickiewicz TL. Primary surgical treatment of anterior cruciate ligament lesions. Am J Sports Med. 1982;10:103–107. doi: 10.1177/036354658201000208. [DOI] [PubMed] [Google Scholar] · DOI
  9. [9]Xie X, Liu X, Chen Z, Yu Y, Peng S, Li Q. A meta-analysis of bone-patellar tendon-bone autograft versus four-strand hamstring tendon autograft for anterior cruciate ligament reconstruction. Knee. 2014 doi: 10.1016/j.knee.2014.11.014. [DOI] [PubMed] [Google Scholar] · DOI
  10. [10]Hu J, Qu J, Xu D, Zhou J, Lu H. Allograft versus autograft for anterior cruciate ligament reconstruction: an up-to-date meta-analysis of prospective studies. Int Orthop. 2013;37(2):311–320. doi: 10.1007/s00264-012-1720-5. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  11. [12]Figueroa D, Figueroa F, Calvo R, Vaisman A, Ahumada X, Arellano S. Platelet-Rich Plasma Use in Anterior Cruciate Ligament Surgery: Systematic Review of the Literature. Arthroscopy. 2015 doi: 10.1016/j.arthro.2014.11.022. [DOI] [PubMed] [Google Scholar] · DOI
  12. [13]Sánchez M, Anitua E, Azofra J, Prado R, Muruzabal F, Andia I. Ligamentization of tendon grafts treated with an endogenous preparation rich in growth factors: gross morphology and histology. Arthroscopy. 2010;26(4):470–480. doi: 10.1016/j.arthro.2009.08.019. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  13. [16]Padulo J, Oliva F, Frizziero A, Maffulli N. Muscles, Ligaments and Tendons Journal. Basic principles and recommendations in clinical and field science research. MLTJ. 2013;4:250–252. [PMC free article] [PubMed] [Google Scholar]
  14. [17]Patel S, Dhillon MS, Aggarwal S, Marwaha N, Jain A. Treatment with platelet-rich plasma is more effective than placebo for knee osteoarthritis: a prospective, double-blind, randomized trial. Am J Sports Med. 2013;41(2):356–364. doi: 10.1177/0363546512471299. [DOI] [PubMed] [Google Scholar] · DOI
  15. [18]Dhillon MS, Behera P, Patel S, Shetty V. Orthobiologics and platelet rich plasma. Indian J Orthop. 2014;48(1):1–9. doi: 10.4103/0019-5413.125477. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  16. [19]Behera P, Dhillon M, Aggarwal S, Marwaha N, Prakash M. Leukocyte-poor platelet-rich plasma versus bupivacaine for recalcitrant lateral epicondylar tendinopathy. J Orthop Surg (Hong Kong) 2015;23(1):6–10. doi: 10.1177/230949901502300102. [DOI] [PubMed] [Google Scholar] · DOI
  17. [20]Sheth U, Simunovic N, Klein G, et al. Efficacy of autologous platelet-rich plasma use for orthopaedic indications: a meta-analysis. J Bone Joint Surg Am. 2012;94(4):298–307. doi: 10.2106/JBJS.K.00154. [DOI] [PubMed] [Google Scholar] · DOI
  18. [21]Kanazawa T, Soejima T, Noguchi K, Tabuchi K, Noyama M, Nakamura K, Shiba N. Tendon-to-bone healing using autologous bone marrow-derived mesenchymal stem cells in ACL reconstruction without a tibial bone tunnel-A histological study. Muscles Ligaments Tendons J. 2014;4(2):201–206. [PMC free article] [PubMed] [Google Scholar]
  19. [22]Lovric V, Kanazawa T, Nakamura Y, Oliver RA, Yu Y, Walsh WR. Effects of Gaps Induced Into the ACL Tendon Graft on Tendon-Bone Healing in a Rodent ACL Reconstruction Model. Muscles Ligaments Tendons J. 2012;1(3):91–99. [PMC free article] [PubMed] [Google Scholar]
  20. [23]Figueroa D, Melean P, Calvo R, et al. Magnetic resonance imaging evaluation of the integration and maturation of semi-tendinosus-gracilis graft in anterior cruciate ligament reconstruction using autologous platelet concentrate. Arthroscopy. 2010;26:1318–1325. doi: 10.1016/j.arthro.2010.02.010. [DOI] [PubMed] [Google Scholar] · DOI
  21. [24]Murray MM, Spindler KP, Abreu E, et al. Collagen-platelet rich plasma hydrogel enhances primary repair of the porcine anterior cruciate ligament. J Orthop Res. 2007;25(1):81–91. doi: 10.1002/jor.20282. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  22. [25]Fallouh L, Nakagawa K, Sasho T, et al. Effects of autologous platelet-rich plasma on cell viability and collagen synthesis in injured human anterior cruciate ligament. J Bone Joint Surg Am. 2010;92(18):2909–2916. doi: 10.2106/JBJS.I.01158. [DOI] [PubMed] [Google Scholar] · DOI · Paper page

Metadata

Authors
Singh Dhillon, M.
Venue
Muscles, Ligaments and Tendons Journal
Publisher
Edikta s.r.l.
Published
2015
Volume
5
Issue
3
Pages
156-61
ISSN
2240-4554 (ISSN-L)
DOI
10.11138/mltj/2015.5.3.156
PMC
PMC4617214
OpenAlex
W2418715866
Cited by
14
References
26
Citations by year
2024: 3 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2
FWCI
1.72
Type
journal article
Language
en
Open access
Locations
  • Muscles Ligaments and Tendons Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Dentistry › Periodontics › Periodontal Regeneration and Treatments
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Tendon Structure and Treatment
  • Health Sciences › Medicine › Surgery › Knee injuries and reconstruction techniques
Keywords
platelet-rich plasma, platelet-poor plasma, anterior cruciate ligament, cell proliferation, cell viability, growth factors, anterior cruciate ligament reconstruction, ligament repair, apoptosis

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CoChat. (2026). Citation check: Can Platelet rich plasma stimulate human ACL growth in culture? A preliminary experience. https://app.cochat.ai/papers/10.11138/mltj/2015.5.3.156
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Singh Dhillon, M. (2015). Can Platelet rich plasma stimulate human ACL growth in culture? A preliminary experience. Muscles, Ligaments and Tendons Journal. https://doi.org/10.11138/mltj/2015.5.3.156