The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Measuring the Contractile Forces of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Arrays of Microposts
Open accessAbstract
Human stem cell-derived cardiomyocytes hold promise for heart repair, disease modeling, drug screening, and for studies of developmental biology. All of these applications can be improved by assessing the contractility of cardiomyocytes at the single cell level. We have developed an in vitro platform for assessing the contractile performance of stem cell-derived cardiomyocytes that is compatible with other common endpoints such as microscopy and molecular biology. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were seeded onto elastomeric micropost arrays in order to characterize the contractile force, velocity, and power produced by these cells. We assessed contractile function by tracking the deflection of microposts beneath an individual hiPSC-CM with optical microscopy. Immunofluorescent staining of these cells was employed to assess their spread area, nucleation, and sarcomeric structure on the microposts. Following seeding of hiPSC-CMs onto microposts coated with fibronectin, laminin, and collagen IV, we found that hiPSC-CMs on laminin coatings demonstrated higher attachment, spread area, and contractile velocity than those seeded on fibronectin or collagen IV coatings. Under optimized conditions, hiPSC-CMs spread to an area of approximately 420 μm2, generated systolic forces of approximately 15 nN/cell, showed contraction and relaxation rates of 1.74 μm/s and 1.46 μm/s, respectively, and had a peak contraction power of 29 fW. Thus, elastomeric micropost arrays can be used to study the contractile strength and kinetics of hiPSC-CMs. This system should facilitate studies of hiPSC-CM maturation, disease modeling, and drug screens as well as fundamental studies of human cardiac contraction.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
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Patient-specific induced pluripotent stem cells as a model for familial dilated cardiomyopathy
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Controlling the contractile strength of engineered cardiac muscle by hierarchal tissue architecture
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Cited by
Sala, L., van Meer, B. J., Tertoolen, L. G. J., Bakkers, J., Bellin, M., Davis, R. P., Denning, C., Dieben, M. A. E., Eschenhagen, T., Giacomelli, E., et al. (2018) · Circulation Research · doi:10.1161/circresaha.117.312067 · Cited by 416
Evolution of strategies to improve preclinical cardiac safety testing
Gintant, G., Sager, P. T., & Stockbridge, N. (2016) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2015.34 · Cited by 407
Denning, C., Borgdorff, V., Crutchley, J., Firth, K. S. A., George, V., Kalra, S., Kondrashov, A., Hoang, M. D., Mosqueira, D., Patel, A., et al. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2015.10.014 · Cited by 293
Engineering Liver Microtissues for Disease Modeling and Regenerative Medicine
Huang, D. (., Gibeley, S. B., Xu, C., Xiao, Y., Celik, O., Ginsberg, H. N., & Leong, K. W. (2020) · Advanced Functional Materials · doi:10.1002/adfm.201909553 · Cited by 65
Gerbin, K. A., Grancharova, T., Donovan-Maiye, R. M., Hendershott, M. C., Anderson, H. G., Brown, J. M., Chen, J., Dinh, S. Q., Gehring, J. L., Johnson, G. R., et al. (2021) · Cell Systems · doi:10.1016/j.cels.2021.05.001 · Cited by 59
Metadata
- Authors
- Rodriguez, M. L., Graham, B. T., Pabon, L. M., Han, S. J., Murry, C. E., & Sniadecki, N. J.
- Venue
- Journal of Biomechanical Engineering
- Publisher
- ASME International
- Published
- 2014-04-10
- Volume
- 136
- Issue
- 5
- Pages
- 051005-051005
- ISSN
- 0148-0731 (ISSN-L)
- 1528-8951
- OpenAlex
- W2039276611
- Cited by
- 162
- References
- 6
- FWCI
- 8.13
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rodriguez, M. L., Graham, B. T., Pabon, L. M., Han, S. J., Murry, C. E., & Sniadecki, N. J. (2014). Measuring the Contractile Forces of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Arrays of Microposts. Journal of Biomechanical Engineering. https://doi.org/10.1115/1.4027145
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