The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Second-harmonic imaging microscopy of living cells
Open accessAbstract
Second harmonic generation (SHG) has been developed in our laboratories as a high-resolution nonlinear optical imaging microscopy for cellular membranes and intact tissues. SHG shares many of the advantageous features for microscopy of another more established nonlinear optical technique: two-photon excited fluorescence (TPEF). Both are capable of optical sectioning to produce three-dimensional images of thick specimens and both result in less photodamage to living tissue than confocal microscopy. SHG is complementary to TPEF in that it uses a different contrast mechanism and is most easily detected in the transmitted light optical path. It can be used to image membrane probes with high membrane specificity and displays extraordinary sensitivity in reporting membrane potential; it also has the ability to image highly ordered structural proteins without any exogenous labels.
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Kleinfeld, D., Mitra, P. P., Helmchen, F., & Denk, W. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15741 · Cited by 832
Cited by
Nanochemistry and Nanomedicine for Nanoparticle-based Diagnostics and Therapy
Chen, G., Roy, I., Yang, C., & Prasad, P. N. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00148 · Cited by 1,499
Campagnola, P. J., & Loew, L. M. (2003) · Nature Biotechnology · doi:10.1038/nbt894 · Cited by 1,326
Self-citationZoumi, A., Yeh, A., & Tromberg, B. J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.172368799 · Cited by 870
3-Dimensional imaging of collagen using second harmonic generation
Cox, G., Kable, E., Jones, A., Fraser, I., Manconi, F., & Gorrell, M. D. (2003) · Journal of Structural Biology · doi:10.1016/s1047-8477(02)00576-2 · Cited by 430
Polarization-Modulated Second Harmonic Generation in Collagen
Stoller, P., Reiser, K. M., Celliers, P. M., & Rubenchik, A. M. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75673-7 · Cited by 400
Metadata
- Authors
- Campagnola, P. J., Clark, H. A., Mohler, W. A., Lewis, A., & Loew, L. M.
- Venue
- Journal of Biomedical Optics
- Publisher
- SPIE-Intl Soc Optical Eng
- Published
- 2001
- Volume
- 6
- Issue
- 3
- Pages
- 277
- ISSN
- 1083-3668 (ISSN-L)
- OpenAlex
- W2120437765
- Cited by
- 244
- References
- 1
- FWCI
- 8.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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