The DOI matches its Crossref record; the references it cites are not checked yet.
Site-directed immobilization of antibody using EDC-NHS-activated protein A on a bimetallic-based surface plasmon resonance chip
Open accessAbstract
The characteristics of a waveguide-coupled bimetallic surface plasmon resonance (WcBiM SPR) sensor using (3-dimethylaminopropyl)-3-ethylcarbodiimide(EDC)-N-hydroxysuccinimide(NHS)-activated protein A was investigated, and the detection of IgG using the EDC-NHS-activated protein A was studied in comparison with protein A and a self-assembled monolayer (SAM). The WcBiM sensor, which has a narrower full width at half maximum (FWHM) and a steeper slope, was selected since it leads to a larger change in the reflectance in the intensity detection mode. A preparation of the EDC-NHS-activated protein A for site-directed immobilization of antibodies was relative easily compared to the engineered protein G and A. In antigen–antibody interactions, the response to IgG at the concentrations of 50, 100, and 150 ng/ml was investigated. The results showed that the sensitivity of the WcBiM sensor using the EDC-NHS-activated protein A, protein A, and SAM was 0.0185 [%/(ng/ml) ], 0.0065 [%/(ng/ml) ], and 0.0101 [%/(ng/ml) ], respectively. The lowest detectable concentrations of IgG with the EDC-NHS-activated protein A, protein A, and SAM were 4.27, 12.83, and 8.24 ng/ml , respectively. Therefore, the increased sensitivity and lower detection capability of the WcBiM SPR chip with the EDC-NHS-activated protein A suggests that it could be used in early diagnosis where the trace level concentrations of biomolecules should be detected.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
The citation lists haven’t been fetched yet
References 23 · Cited by 34
Research works printed but not linked: 19
- 10.2147/NSA.S13465 · DOI
- 10.1124/pr.59.1.1 · DOI
- 10.1016/j.nantod.2011.02.001 · DOI
- 10.1016/j.tet.2012.05.018 · DOI
- 10.3938/jkps.62.475 · DOI
- 10.1016/j.bios.2008.03.011 · DOI
- 10.1166/jno.2011.1179 · DOI
- 10.1016/j.talanta.2013.06.034 · DOI
- 10.1016/S0925-4005(98)00321-9 · DOI
- 10.1080/02726340801921650 · DOI
- 10.1007/s13320-011-0051-2 · DOI
- 10.1016/j.mee.2013.02.088 · DOI
- 10.1002/(ISSN)1097-0290 · DOI
- 10.1111/ejb.1977.78.issue-2 · DOI
- 10.1016/j.bios.2009.01.026 · DOI
- 10.3390/diagnostics2030023 · DOI
- 10.1021/ac0619231 · DOI
- 10.1039/c3an36498d · DOI
- 10.1186/1556-276X-8-344 · DOI
Metadata
- Authors
- Sohn, Y.-S., & Lee, Y. K.
- Affiliations
- Daegu Catholic University (KR)
- Venue
- Journal of Biomedical Optics
- Publisher
- SPIE-Intl Soc Optical Eng
- Published
- 2014-01-13
- Volume
- 19
- Issue
- 05
- Pages
- 1-7
- ISSN
- 1083-3668 (ISSN-L)
- 1560-2281
- OpenAlex
- W1967830457
- Cited by
- 34
- References
- 23
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 3 · 2014: 1
- FWCI
- 1.37
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Biomedical Optics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea funded by the Ministry of Education (2013R1A1A2010028)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Plasmonic and Surface Plasmon Research
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Advanced Fiber Optic Sensors
- Keywords
- surface plasmon resonance, antibody immobilization, protein A, self-assembled monolayers, IgG detection, antigen-antibody reaction, early diagnosis
- MeSH terms
- Carbodiimides, Equipment Design, Immunoglobulin G, Staphylococcal Protein A, Succinimides, Biosensing Techniques, Surface Plasmon Resonance, Antibodies, Immobilized
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Site-directed immobilization of antibody using EDC-NHS-activated protein A on a bimetallic-based surface plasmon resonance chip. https://app.cochat.ai/papers/10.1117/1.jbo.19.5.051209
- The paper
- Sohn, Y.-S., & Lee, Y. K. (2014). Site-directed immobilization of antibody using EDC-NHS-activated protein A on a bimetallic-based surface plasmon resonance chip. Journal of Biomedical Optics. https://doi.org/10.1117/1.jbo.19.5.051209
