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Spot Urine Concentrations Should Not Be Used for Hydration Assessment: A Methodology Review
Abstract
A common practice in sports science is to assess hydration status using the concentration of a single spot urine collection taken at any time of day for comparison against concentration (specific gravity, osmolality, color) thresholds established from first morning voids. There is strong evidence that this practice can be confounded by fluid intake, diet, and exercise, among other factors, leading to false positive/negative assessments. Thus, the purpose of this paper is to provide a simple explanation as to why this practice leads to erroneous conclusions and should be curtailed in favor of consensus hydration assessment recommendations.
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In the citing paper
“Spot urine samples at other times of the day can be confounded by fluid intake, exercise, and food intake and may not be accurate indicators of hydration status.”
From National Athletic Trainers' Association Position Statement: Fluid Replacement for the Physically Active (2017) · cites this paper as [137]
In the citing paper
“When this is not feasible, ATs should know that urine analysis may confound the clinical decision-making process and that body mass assessment provides more reliable information. 137”
From National Athletic Trainers' Association Position Statement: Fluid Replacement for the Physically Active (2017) · cites this paper as [137]
In the citing paper
“An important consideration when assessing hydration is that urine volume and concentration are affected not only by fluid loss but also by activity, diet, and especially fluid intake. 137”
From National Athletic Trainers' Association Position Statement: Fluid Replacement for the Physically Active (2017) · cites this paper as [137]
In the citing paper
“As long as the first morning measurements of urine concentration are used to assess hydration, any one of them serves as an acceptable complement to body mass and thirst observations for assessing hydration. 137”
From National Athletic Trainers' Association Position Statement: Fluid Replacement for the Physically Active (2017) · cites this paper as [137]
In the citing paper
“The most stable value, which provides a valid and reliable indication of hydration status, is the first morning assessment.”
From National Athletic Trainers' Association Position Statement: Fluid Replacement for the Physically Active (2017) · cites this paper as [137]
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Cited by
McDermott, B. P., Anderson, S. A., Armstrong, L. E., Casa, D. J., Cheuvront, S. N., Cooper, L., Kenney, W. L., O'Connor, F. G., & Roberts, W. O. (2017) · Journal of Athletic Training · doi:10.4085/1062-6050-52.9.02 · Cited by 438
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Metadata
- Authors
- Cheuvront, S. N., Kenefick, R. W., & Zambraski, E. J.
- Venue
- International Journal of Sport Nutrition and Exercise Metabolism
- Publisher
- Human Kinetics
- Published
- 2015-06
- Volume
- 25
- Issue
- 3
- Pages
- 293-297
- ISSN
- 1526-484X
- 1543-2742
- Cited by
- 66
- References
- 0
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- CoChat. (2026). Citation check: Spot Urine Concentrations Should Not Be Used for Hydration Assessment: A Methodology Review. https://app.cochat.ai/papers/10.1123/ijsnem.2014-0138
- The paper
- Cheuvront, S. N., Kenefick, R. W., & Zambraski, E. J. (2015). Spot Urine Concentrations Should Not Be Used for Hydration Assessment: A Methodology Review. International Journal of Sport Nutrition and Exercise Metabolism. https://doi.org/10.1123/ijsnem.2014-0138
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.