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Cited by 86Epigenetics and DNA MethylationGenomics and Chromatin DynamicsDNA and Nucleic Acid Chemistry

Measuring quantitative effects of methylation on transcription factor–DNA binding affinity

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Zuo, Zheng, Roy, Basab, Chang, Yiming Kenny, Granas, David, Stormo, Gary D.·2017·Science Advances
DOI 10.1126/sciadv.aao1799PMC PMC5694663
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Abstract

We describe a new method for measuring the effects of epigenetic marks on protein-DNA interactions.

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  • Hypermethylation of DMR_394 may disrupt binding of regulatory proteins and adversely affect the organization and regulation of nearby genes.

    “Given that the affinity of many transcription factors, including CTCF31, is negatively impacted by methylation of their binding sites32, it is possible that hypermethylation of DMR_394 disrupts binding of regulatory proteins and adversely affects the organization and regulation of genes nearby.”

    From Integrated analysis of behavioral, epigenetic, and gut microbiome analyses in AppNL-G-F, AppNL-F, and wild type mice (2021) · cites this paper as [31]

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References 49 · Cited by 86

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Authors
Zuo, Z., Roy, B., Chang, Y. K., Granas, D., & Stormo, G. D.
Affiliations
Washington University in St. Louis (US)
Corresponding author
  • Zuo, Zheng
  • Roy, Basab
  • Stormo, Gary D.
ORCID
Venue
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Published
2017-11-03
Volume
3
Issue
11
Pages
eaao1799-eaao1799
ISSN
2375-2548 (ISSN-L)
DOI
10.1126/sciadv.aao1799
PMC
PMC5694663
OpenAlex
W2770182290
Cited by
86
References
49
Citations by year
2026: 3 · 2025: 6 · 2024: 9 · 2023: 14 · 2022: 16 · 2021: 10 · 2020: 8 · 2019: 14 · 2018: 6
FWCI
3.13
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Science Advances (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Digital Commons@Becker (Washington University School of Medicine) (submittedVersion)
Funders
National Institutes of Health (award335530, HG000249)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
DNA methylation, CpG methylation, transcription factor binding affinity, epigenetic regulation, fluorescence anisotropy, competitive binding assay, DNA-binding proteins, gene expression regulation
MeSH terms
Patched-1 Receptor, CCCTC-Binding Factor, Animals, DNA, Fluorescence Polarization, Protein Binding, Recombinant Proteins, Repressor Proteins, Transcription Factors, Homeodomain Proteins, CpG Islands, DNA Methylation, Mice

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