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Cited by 973D Printing in Biomedical ResearchPancreatic function and diabetesTissue Engineering and Regenerative Medicine

Organoid microphysiological system preserves pancreatic islet function within 3D matrix

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Patel, S. N., Ishahak, M., Chaimov, D., Velraj, A., LaShoto, D., Hagan, D. W., Buchwald, P., Phelps, E. A. +2 more·2021·Science Advances
DOI 10.1126/sciadv.aba5515PMC PMC7880596
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Abstract

A customized microphysiological system supports the perifusion and in situ multiparametric assessment of 3D islet organoids.

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  • Relying on active fluid flow rather than passive diffusion for nutrient transport can greatly enhance the viability and performance of islets within a macroencapsulation device.

    “this study highlights that relying on active fluid flow rather than passive diffusion for nutrient transport can greatly enhance the viability and performance of islets within a macroencapsulation device.”

    From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [95]

    The Results sections on rodent islet viability and human islet culture report better viability and preserved insulin responses with continuous flow than with static culture. The Discussion says improved nutrient delivery is a likely explanation, but notes that flow may have other effects. The experiments used an in vitro microphysiological chip with alginate-embedded islets, not a macroencapsulation device, so that device-specific extension is not established. (LLM, conf=0.86)

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Authors
Patel, S. N., Ishahak, M., Chaimov, D., Velraj, A., LaShoto, D., Hagan, D. W., Buchwald, P., Phelps, E. A., Agarwal, A., & Stabler, C. L.
Affiliations
  • University of Florida (US)
  • University of Miami (US)
  • University of Florida Health (US)
Corresponding author
  • Patel, S. N.
  • Ishahak, M.
  • Agarwal, A.
  • Stabler, C. L.
ORCID
Venue
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Published
2021-02-12
Volume
7
Issue
7
ISSN
2375-2548 (ISSN-L)
DOI
10.1126/sciadv.aba5515
PMC
PMC7880596
OpenAlex
W3128226077
Cited by
97
References
69
Citations by year
2026: 7 · 2025: 22 · 2024: 14 · 2023: 27 · 2022: 21 · 2021: 6
FWCI
5.17
Type
journal article
Language
en
Open access
Licence
Locations
  • Science Advances (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (UC4 DK104208, UG3 DK122638)
  • Human Islet Research Network (F31DK118860)
  • Integrated Islet Distribution Program (IIDP) at City of Hope (2UC4DK098085)
  • Human Islet Cell Processing Facility at the Diabetes Research Institute
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
pancreatic islets, organoids, microphysiological systems, dynamic culture, hypoxia, insulin secretion, organoid viability, computational modeling, high-content imaging

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