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Cited by 9Genomics and Chromatin DynamicsDNA Repair MechanismsMicrotubule and mitosis dynamics

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The synaptonemal complex aligns meiotic chromosomes by wetting

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Gordon, Spencer G., Rodriguez, Alyssa A., Gu, Yajie, Corbett, Kevin D., Lee, Chiu Fan, Rog, Ofer·2025·Science Advances
DOI 10.1126/sciadv.adt5675PMC PMC11864179

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Abstract

During meiosis, the parental chromosomes are drawn together to enable exchange of genetic information. Chromosomes are aligned through the assembly of a conserved interface, the synaptonemal complex, composed of a central region that forms between two parallel chromosomal backbones called axes. Here, we identify the axis-central region interface in C. elegans , containing a conserved positive patch on the axis component HIM-3 and the negative C terminus of the central region protein SYP-5. Crucially, the canonical ultrastructure of the synaptonemal complex is altered upon weakening this interface using charge-reversal mutations. We developed a thermodynamic model that recapitulates our experimental observations, indicating that the liquid-like central region can assemble by wetting the axes without active energy consumption. More broadly, our data show that condensation drives tightly regulated nuclear reorganization during sexual reproduction.

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Metadata

Authors
Gordon, S. G., Rodriguez, A. A., Gu, Y., Corbett, K. D., Lee, C. F., & Rog, O.
Affiliations
  • University of Utah (US)
  • University of California San Diego (US)
  • Imperial College London (GB)
Corresponding author
Rog, Ofer
ORCID
Venue
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Published
2025-02-28
Volume
11
Issue
9
Pages
eadt5675-eadt5675
ISSN
2375-2548 (ISSN-L)
DOI
10.1126/sciadv.adt5675
PMC
PMC11864179
OpenAlex
W4407948762
Cited by
9
References
67
Citations by year
2026: 5 · 2025: 2
FWCI
2.09
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Science Advances (publishedVersion, cc-by-nc)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by-nc)
  • eScholarship (California Digital Library) (submittedVersion, cc-by-nc)
Funders
  • National Institutes of Health (OD010440, P40OD010440)
  • National Institute of General Medical Sciences (R35GM144121, K12GM068524, R35GM128804)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
synaptonemal complex, wettability, liquid-liquid phase separation, charge reversal mutations, thermodynamic modeling, condensin, nuclear reorganization, Caenorhabditis elegans, meiosis

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