Structural basis of gating by the outer membrane transporter FecA
Abstract
Siderophore-mediated acquisition systems facilitate iron uptake. We present the crystallographic structure of the integral outer membrane receptor FecA from Escherichia coli with and without ferric citrate at 2.5 and 2.0 angstrom resolution. FecA is composed of three distinct domains: the barrel, plug, and NH2-terminal extension. Binding of ferric citrate triggers a conformational change of the extracellular loops that close the external pocket of FecA. Ligand-induced allosteric transitions are propagated through the outer membrane by the plug domain, signaling the occupancy of the receptor in the periplasm. These data establish the structural basis of gating for receptors dependent on the cytoplasmic membrane protein TonB. By compiling available data for this family of receptors, we propose a mechanism for the energy-dependent transport of siderophores.
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Fiu mimics the induced fit binding mechanisms of FepA and FecA as they close around their iron complexes.
“However, in this respect, Fiu mimics the induced fit binding mechanisms of FepA ( Scott et al., 2002 ; Smallwood et al., 2014 ) and FecA ( Ferguson et al., 2002 ) as they close around their iron complexes.”
From Specificity and mechanism of TonB-dependent ferric catecholate uptake by Fiu (2024) · cites this paper as Ferguson et al., 2002
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References 37 · Cited by 373
- Authors
- Ferguson, A. D., Chakraborty, R., Smith, B. S., Esser, L., van der Helm, D., & Deisenhofer, J.
- Affiliations
- Howard Hughes Medical Institute (US)
- East Tennessee State University (US)
- National Institutes of Health (US)
- Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, OK 73019, USA.
- Corresponding author
- Deisenhofer, Johann
- Venue
- Science
- Published
- 2002-03
- Volume
- 295
- Issue
- 5560
- Pages
- 1715-1719
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2108623931
- Cited by
- 373
- References
- 37
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 6 · 2023: 4 · 2022: 6 · 2021: 7 · 2020: 8 · 2019: 11 · 2018: 5 · 2017: 9 · 2016: 7 · 2015: 8 · 2014: 12 · 2013: 12 · 2012: 15
- FWCI
- 22.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- FecA, outer membrane receptor, siderophore uptake, ferric citrate, TonB-dependent receptors, allosteric gating, conformational change, plug domain, Escherichia coli, iron acquisition, crystal structure, energy-dependent transport
- MeSH terms
- Adsorption, Bacterial Outer Membrane Proteins, Bacterial Proteins, Binding Sites, Biological Transport, Active, Carrier Proteins, Cell Membrane, Ferric Compounds, Hydrogen Bonding, Ligands, Membrane Proteins, Models, Molecular, Protein Binding, Protein Conformation, Receptors, Cell Surface, Ion Channel Gating, Siderophores, Protein Structure, Secondary, Protein Structure, Tertiary, Protein Folding, Crystallography, X-Ray, Escherichia coli Proteins, Static Electricity
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