The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Capturing Chromosome Conformation
Abstract
We describe an approach to detect the frequency of interaction between any two genomic loci. Generation of a matrix of interaction frequencies between sites on the same or different chromosomes reveals their relative spatial disposition and provides information about the physical properties of the chromatin fiber. This methodology can be applied to the spatial organization of entire genomes in organisms from bacteria to human. Using the yeast Saccharomyces cerevisiae , we could confirm known qualitative features of chromosome organization within the nucleus and dynamic changes in that organization during meiosis. We also analyzed yeast chromosome III at the G 1 stage of the cell cycle. We found that chromatin is highly flexible throughout. Furthermore, functionally distinct AT- and GC-rich domains were found to exhibit different conformations, and a population-average 3D model of chromosome III could be determined. Chromosome III emerges as a contorted ring.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“As applied to enhancers in vertebrate animals, this model received strong confirmation after the development of methods for studying the spatial organization of the genome using the proximity ligation technique [29].”
From Enhancer Function in the 3D Genome (2023) · cites this paper as [29]
Citations
Cites
Statistical mechanics of chain molecules
Flory, P. J., & Volkenstein, M. V. (1969) · Biopolymers · doi:10.1002/bip.1969.360080514 · Cited by 6,449
Chromosome territories, nuclear architecture and gene regulation in mammalian cells
Cremer, T. J., & Cremer, C. G. (2001) · Nature Reviews Genetics · doi:10.1038/35066075 · Cited by 2,339
Röntgenuntersuchung gelöster Fadenmoleküle
Kratky, O., & Porod, G. (1949) · Recueil des Travaux Chimiques des Pays-Bas · doi:10.1002/recl.19490681203 · Cited by 1,994
Meiotic Chromosomes: Integrating Structure and Function
Zickler, D., & Kleckner, N. E. (1999) · Annual Review of Genetics · doi:10.1146/annurev.genet.33.1.603 · Cited by 1,256
Self-citationChromatin Structure and Function
Nicolini, C. A. (1979) · Springer eBooks · doi:10.1007/978-1-4684-0973-4 · Cited by 1,035
Hunter, N. M., & Kleckner, N. E. (2001) · Cell · doi:10.1016/s0092-8674(01)00430-5 · Cited by 663
Self-citationvan Holde, K. E. (1989) · Springer series in molecular biology · doi:10.1007/978-1-4612-3490-6 · Cited by 654
THE LEPTOTENE-ZYGOTENE TRANSITION OF MEIOSIS
Zickler, D., & Kleckner, N. E. (1998) · Annual Review of Genetics · doi:10.1146/annurev.genet.32.1.619 · Cited by 552
Self-citationThe yeast genome project: what did we learn?
Dujon, B. (1996) · Trends in Genetics · doi:10.1016/0168-9525(96)10027-5 · Cited by 547
Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae
Gerton, J. L., DeRisi, J. L., Shroff, R., Lichten, M., Brown, P. O., & Petes, T. D. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.21.11383 · Cited by 481
Research works printed but not linked: 3
- Cimbora D. M., Groudine M., Cell 104, 643 (2001).
- Supplementary details of experimental procedures are available on Science Online at www.sciencemag.org/cgi/content/full/295/5558/1306/DC1.
- Holmquist G. P., Am. J. Hum. Genet. 51, 17 (1992).
Cited by
Sorted by citations
A 3D Map of the Human Genome at Kilobase Resolution Reveals Principles of Chromatin Looping
Rao, S. S. P., Huntley, M. H., Durand, N. C., Stamenova, E. K., Bochkov, I. D., Robinson, J. T., Sanborn, A. L., Machol, I., Omer, A. D., Lander, E. S., et al. (2014) · Cell · doi:10.1016/j.cell.2014.11.021 · Cited by 9,212
Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome
Lieberman-Aiden, E., van Berkum, N. L., Williams, L., Imakaev, M., Ragoczy, T., Telling, A., Amit, I., Lajoie, B. R., Sabo, P. J., Dorschner, M. O., et al. (2009) · Science · doi:10.1126/science.1181369 · Cited by 8,898
Self-citationMerqury: reference-free quality, completeness, and phasing assessment for genome assemblies
Rhie, A., Walenz, B. P., Koren, S., & Phillippy, A. M. (2020) · Genome Biology · doi:10.1186/s13059-020-02134-9 · Cited by 3,643
Histone methylation: a dynamic mark in health, disease and inheritance
Greer, E. L., & Shi, Y. (2012) · Nature Reviews Genetics · doi:10.1038/nrg3173 · Cited by 2,241
Three-dimensional folding and functional organization principles of the Drosophila genome
Sexton, T., Yaffe, E., Kenigsberg, E., Bantignies, F., Leblanc, B., Hoichman, M., Parrinello, H., Tanay, A., & Cavalli, G. (2012) · Cell · doi:10.1016/j.cell.2012.01.010 · Cited by 1,983
Metadata
- Authors
- Dekker, J., Rippe, K., Dekker, M., & Kleckner, N.
- Affiliations
- Harvard University (US)
- German Cancer Research Center (DE)
- 2e Oosterparklaan 272, 3544 AX Utrecht, Netherlands.
- Corresponding author
- Dekker, Job
- Venue
- Science
- Published
- 2002-02-15
- Volume
- 295
- Issue
- 5558
- Pages
- 1306-1311
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2073026107
- Cited by
- 3,652
- References
- 37
- Citations by year
- 2026: 94 · 2025: 123 · 2024: 161 · 2023: 174 · 2022: 190 · 2021: 228 · 2020: 280 · 2019: 263 · 2018: 260 · 2017: 208 · 2016: 249 · 2015: 221 · 2014: 165 · 2013: 166 · 2012: 190
- FWCI
- 9.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
- Funders
- National Institute of General Medical Sciences (GM-44794, GM 25326, R01GM044794)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- chromosome conformation, 3D genome organization, chromatin fiber, Saccharomyces cerevisiae, meiosis
- MeSH terms
- Cell Fractionation, Cell Nucleus, Centromere, Chromatin, Cross-Linking Reagents, Formaldehyde, Mathematics, Meiosis, Mitosis, Protein Conformation, Saccharomyces cerevisiae, Deoxyribonuclease EcoRI, Chromosomes, Fungal, Polymerase Chain Reaction, G1 Phase, Telomere, Genome, Fungal, AT Rich Sequence, GC Rich Sequence
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
