The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Experimental Identification of Downhill Protein Folding
Abstract
Theory predicts the existence of barrierless protein folding. Without barriers, folding should be noncooperative and the degree of native structure should be coupled to overall protein stability. We investigated the thermal unfolding of the peripheral subunit binding domain from Escherichia coli 's 2-oxoglutarate dehydrogenase multienzyme complex (termed BBL) with a combination of spectroscopic techniques and calorimetry. Each technique probed a different feature of protein structure. BBL has a defined three-dimensional structure at low temperatures. However, each technique showed a distinct unfolding transition. Global analysis with a statistical mechanical model identified BBL as a downhill-folding protein. Because of BBL's biological function, we propose that downhill folders may be molecular rheostats, in which effects could be modulated by altering the distribution of an ensemble of structures.
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Cites
Principles that Govern the Folding of Protein Chains
Anfinsen, C. B. (1973) · Science · doi:10.1126/science.181.4096.223 · Cited by 7,096
Funnels, pathways, and the energy landscape of protein folding: A synthesis
Bryngelson, J. D., Onuchic, J. N., Socci, N. D., & Wolynes, P. G. (1995) · Proteins: Structure, Function, and Bioinformatics · doi:10.1002/prot.340210302 · Cited by 2,648
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Dill, K. A., Ozkan, S. B., Shell, M. S., & Weikl, T. R. (2008) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.37.092707.153558 · Cited by 1,163
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Metadata
- Authors
- Garcia-Mira, M. M., Sadqi, M., Fischer, N., Sanchez-Ruiz, J. M., & Muñoz, V.
- Venue
- Science
- Published
- 2002-12-13
- Volume
- 298
- Issue
- 5601
- Pages
- 2191-2195
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2096599403
- Cited by
- 307
- References
- 2
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- Garcia-Mira, M. M., Sadqi, M., Fischer, N., Sanchez-Ruiz, J. M., & Muñoz, V. (2002). Experimental Identification of Downhill Protein Folding. Science. https://doi.org/10.1126/science.1077809
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