Nervous System Targets of RNA Editing Identified by Comparative Genomics
Abstract
An unknown number of precursor messenger RNAs undergo genetic recoding by modification of adenosine to inosine, a reaction catalyzed by the adenosine deaminases acting on RNA (ADARs). Discovery of these edited transcripts has always been serendipitous. Using comparative genomics, we identified a phylogenetic signature of RNA editing. We report the identification and experimental verification of 16 previously unknown ADAR target genes in the fruit fly Drosophila and one in humans—more than the sum total previously reported. All of these genes are involved in rapid electrical and chemical neurotransmission, and many of the edited sites recode conserved and functionally important amino acids. These results point to a pivotal role for RNA editing in nervous system function.
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The apparent connection with RNA editing occurs also in insects; the RNA-edited potassium channel genes shaker, ether-a-go-go, and slowpoke each overlap four or more HCEs.
“The apparent connection with RNA editing occurs also in insects; the RNA-edited potassium channel genes shaker, ether-a-go-go, and slowpoke (Hoopengardner et al. 2003) each overlap four or more HCEs (see related observations by Glazov et al. 2005).”
From Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes (2005) · cites this paper as Hoopengardner et al. 2003
A recently identified RNA-editing site in KCNA1 also corresponds to an HCE, one of the top 100 by log-odds score.
“A recently identified RNA-editing site in KCNA1 (Hoopengardner et al. 2003) also corresponds to an HCE, one of the top 100 by log-odds score.”
From Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes (2005) · cites this paper as Hoopengardner et al. 2003
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Evolution of voltage-gated ion channels at the emergence of Metazoa
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- Authors
- Hoopengardner, B., Bhalla, T., Staber, C., & Reenan, R.
- Affiliations
- UConn Health (US)
- Corresponding author
- Hoopengardner, Barry
- Bhalla, Tarun
- Reenan, Robert
- Venue
- Science
- Published
- 2003-08-08
- Volume
- 301
- Issue
- 5634
- Pages
- 832-836
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1985317176
- Cited by
- 376
- References
- 0
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 9 · 2023: 5 · 2022: 7 · 2021: 9 · 2020: 15 · 2019: 5 · 2018: 9 · 2017: 15 · 2016: 12 · 2015: 20 · 2014: 13 · 2013: 18 · 2012: 31
- FWCI
- 9.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM062291)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
- Keywords
- RNA editing, adenosine deaminases acting on RNA, ADAR, A-to-I RNA editing, nervous system, neurotransmission, Drosophila, human, comparative genomics
- MeSH terms
- Adenosine, Adenosine Deaminase, Animals, Base Sequence, Drosophila, Drosophila melanogaster, Humans, Inosine, Ion Channels, Molecular Sequence Data, Nervous System, Synaptic Transmission, Phylogeny, Synapses, Potassium Channels, Ion Channel Gating, RNA-Binding Proteins, Genes, Insect, RNA Editing, Reverse Transcriptase Polymerase Chain Reaction, Genomics
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