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NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression
Abstract
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
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Citations
Cited by
Clore, G. M., & Iwahara, J. (2009) · Chemical Reviews · doi:10.1021/cr900033p · Cited by 782
Structural basis for signal-sequence recognition by the translocase motor SecA as determined by NMR.
Gelis, I., Bonvin, A. M. J. J., Keramisanou, D., Koukaki, M., Gouridis, G., Karamanou, S., Economou, A., & Kalodimos, C. G. (2007) · Cell · doi:10.1016/j.cell.2007.09.039 · Cited by 433
Engineering Escherichia coli for production of functionalized terpenoids using plant P450s
Chang, M. C. Y., Eachus, R. A., Trieu, W., Ro, D.-K., & Keasling, J. D. (2007) · Nature Chemical Biology · doi:10.1038/nchembio875 · Cited by 364
Protein Design: From the Aspect of Water Solubility and Stability
Qing, R., Hao, S., Smorodina, E., Jin, D., Zalevsky, A., & Zhang, S. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00757 · Cited by 312
Jia, B., & Jeon, C. O. (2016) · Open Biology · doi:10.1098/rsob.160196 · Cited by 295
Metadata
- Authors
- Roosild, T. P., Greenwald, J., Vega, M., Castronovo, S., Riek, R., & Choe, S.
- Venue
- Science
- Published
- 2005-02-25
- Volume
- 307
- Issue
- 5713
- Pages
- 1317-1321
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2004079991
- Cited by
- 247
- References
- 0
- FWCI
- 7.14
- Type
- journal article
- Language
- en
- Open access
- closed
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- Roosild, T. P., Greenwald, J., Vega, M., Castronovo, S., Riek, R., & Choe, S. (2005). NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression. Science. https://doi.org/10.1126/science.1106392
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