The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Global Topology Analysis of the Escherichia coli Inner Membrane Proteome
Abstract
The protein complement of cellular membranes is notoriously resistant to standard proteomic analysis and structural studies. As a result, membrane proteomes remain ill-defined. Here, we report a global topology analysis of the Escherichia coli inner membrane proteome. Using C-terminal tagging with the alkaline phosphatase and green fluorescent protein, we established the periplasmic or cytoplasmic locations of the C termini for 601 inner membrane proteins. By constraining a topology prediction algorithm with this data, we derived high-quality topology models for the 601 proteins, providing a firm foundation for future functional studies of this and other membrane proteomes. We also estimated the overexpression potential for 397 green fluorescent protein fusions; the results suggest that a large fraction of all inner membrane proteins can be produced in sufficient quantities for biochemical and structural work.
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1 retracted
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RetractedBasic local alignment search tool
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Self-citationX-ray structure of a ClC chloride channel at 3.0 Å reveals the molecular basis of anion selectivity
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X-ray structure of a protein-conducting channel
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Structure of a Glycerol-Conducting Channel and the Basis for Its Selectivity
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Self-citationMechanism of Ammonia Transport by Amt/MEP/Rh: Structure of AmtB at 1.35 Å
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A Genetic Approach to Analyzing Membrane Protein Topology
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Cited by
von Heijne, G. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2063 · Cited by 538
Self-citationGlobal Functional Atlas of Escherichia coli Encompassing Previously Uncharacterized Proteins
Hu, P., Janga, S. C., Babu, M., Díaz-Mejía, J. J., Butland, G., Yang, W., Pogoutse, O., Guo, X., Phanse, S., Wong, P., et al. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000096 · Cited by 387
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Self-citationProteomics of Integral Membrane ProteinsTheory and Application
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Comparative Functional Analysis of the Caenorhabditis elegans and Drosophila melanogaster Proteomes
Schrimpf, S. P., Weiss, M., Reiter, L., Ahrens, C. H., Jovanovic, M., Malmström, J., Brunner, E., Mohanty, S., Lercher, M. J., Hunziker, P. E., et al. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000048 · Cited by 229
Metadata
- Authors
- Daley, D. O., Rapp, M., Granseth, E., Melén, K., Drew, D., & von Heijne, G.
- Venue
- Science
- Published
- 2005-05-27
- Volume
- 308
- Issue
- 5726
- Pages
- 1321-1323
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2058658881
- Cited by
- 490
- References
- 21
- FWCI
- 24.03
- Type
- journal article
- Language
- en
- Open access
- closed
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- Daley, D. O., Rapp, M., Granseth, E., Melén, K., Drew, D., & von Heijne, G. (2005). Global Topology Analysis of the Escherichia coli Inner Membrane Proteome. Science. https://doi.org/10.1126/science.1109730
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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