Variable Control of Ets-1 DNA Binding by Multiple Phosphates in an Unstructured Region

Pufall, Miles A.; Lee, Gregory M.; Nelson, Mary L.; Kang, Hyun-Seo; Velyvis, Algirdas; Kay, Lewis E.; McIntosh, Lawrence P.; Graves, Barbara J.

doi:10.1126/science.1111915

Variable Control of Ets-1 DNA Binding by Multiple Phosphates in an Unstructured Region

Cell signaling that culminates in posttranslational modifications directs protein activity. Here we report how multiple Ca 2+ -dependent phosphorylation sites within the transcription activator Ets-1 act additively to produce graded DNA binding affinity. Nuclear magnetic resonance spectroscopic a…